Over the past two decades, bioorthogonal chemistry has undergone a remarkable development, challenging traditional assumptions in biology and medicine. Recent advancements in the design of probes tailored for bioorthogonal applications have met the increasing demand for precise imaging, facilitating the exploration of complex biological systems. These state-of-the-art probes enable highly sensitive, low background, in situ imaging of biological species and events within live organisms, achieving resolutions comparable to the size of the biomolecule under investigation. This review provides a comprehensive examination of various categories of bioorthogonally activated in situ fluorescent labels. It highlights the intricate design and benefits of bioorthogonal chemistry for precise in situ imaging, while also discussing future prospects in this rapidly evolving field.

中文翻译：

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用于精确荧光成像的生物正交激活探针


在过去的二十年里，生物正交化学经历了显着的发展，挑战了生物学和医学的传统假设。为生物正交应用量身定制的探针设计的最新进展满足了对精确成像日益增长的需求，促进了复杂生物系统的探索。这些最先进的探针能够对活体生物体内的生物物种和事件进行高灵敏度、低背景的原位成像，达到与所研究的生物分子大小相当的分辨率。本综述对各种类别的生物正交激活原位荧光标记进行了全面检查。它强调了生物正交化学在精确原位成像方面的复杂设计和优势，同时还讨论了这个快速发展的领域的未来前景。