We are grateful to the reviewers for their globally positive evaluation and the very constructive criticism and suggestions they made. In particular, we acknowledge their request for a detailed discussion of the lessons the community can learn in view of devising new and better performing fluorescent mutants of AR-3. We have now addressed this question in a new section entitled "Engineering Aspects". The second major request from reviewer 1 concerned the conclusion section, which should focus on highlighting the outcome and possible implications of our work for future research in this area. In response to this, we have fully re-organised the conclusion section and moved the paragraphs discussing and summarising the main results to the relevant sections 2 and 3. Other items and questions raised by the reviewers were addressed in detail and led to minor changes (see detailed point-by-point response). In conclusion, we hope that the new version convinces the reviewers, and that the manuscript will be accepted for publication in Chemical Sciences.

中文翻译：

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Archaerhodopsin 3 是高荧光光遗传学报告基因工程的理想模板


我们感谢审稿人在全球范围内的积极评价以及他们提出的非常建设性的批评和建议。特别是，我们承认他们要求详细讨论社区在设计新的和性能更好的 AR-3 荧光突变体方面可以吸取的经验教训。我们现在在题为“工程方面”的新章节中解决了这个问题。审稿人 1 的第二个主要要求涉及结论部分，该部分应侧重于强调我们工作的结果和对该领域未来研究的可能影响。为此，我们完全重新组织了结论部分，并将讨论和总结主要结果的段落移至相关的第 2 节和第 3 节。审稿人提出的其他项目和问题得到了详细解决，并导致了细微的更改（参见详细的逐点回复）。总之，我们希望新版本能说服审稿人，并希望该手稿能够被接受在《化学科学》杂志上发表。