Refining lignocellulose into fermentable monosaccharides remains a challenging endeavor. In this study, we employed a novel deep eutectic solvent (DES) composed of 1-tetradecyl-3-carboxymethylimidazolium bromide (C14CIMBr) and lactic acid (LA) for the pretreatment of Moringa oleifera Lam. husk (M. oleifera husk), aiming to enhance lignocellulose fractionation. The optimal conditions identified were 165 °C, 43.9 min, and a molar ratio of C14CIMBr to LA at 1:4.8, resulting in the removal of 93.8% of lignin and 96.8% of hemicellulose. Following enzymatic hydrolysis of the pretreated M. oleifera husk, yields of glucose and xylose reached 94.3% and 86.1%, respectively, representing significant increases by factors of 7.0-fold and 10.3-fold compared to untreated M. oleifera husk. Molecular simulations indicated that the novel DES effectively disrupts the original internal hydrogen bonding network while reconstructing new DES-lignocellulosic hydrogen bonds, it also demonstrates excellent solubilization capabilities for lignin cleavage products. Finally, the yield of regenerated lignin reached 81.3%, exhibiting notable bactericidal activity under blue light irradiation.

中文翻译：

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促进辣木的木质纤维素分级分离 Lam。用新型 1-十四烷基-3-羧甲基咪唑基溴化物基深共熔溶剂预处理


将木质纤维素提炼成可发酵的单糖仍然是一项具有挑战性的工作。在本研究中，我们采用了一种由 1-十四烷基-3-羧甲基咪唑溴化物 （C14CIMBr） 和乳酸 （LA） 组成的新型低共熔溶剂 （DES） 对辣木油茶进行预处理。外壳 （M. oleifera husk），旨在增强木质纤维素分馏。确定的最佳条件为 165 °C、43.9 分钟和 C14CIMBr 与 LA 的摩尔比为 1：4.8，导致去除 93.8% 的木质素和 96.8% 的半纤维素。酶解预处理后的油分杆菌壳后，葡萄糖和木糖的产量分别达到 94.3% 和 86.1%，与未处理的油分杆菌壳相比，产量显著提高了 7.0 倍和 10.3 倍。分子模拟表明，新型 DES 在重建新的 DES-木质素纤维素氢键的同时，有效地破坏了原始的内部氢键，它还表现出对木质素裂解产物的优异增溶能力。最后，再生木质素的收率达到 81.3%，在蓝光照射下表现出显著的杀菌活性。