Solid phase extraction (SPE) using traditional column materials offers several advantages, but its ability to simultaneously extract and purify commonly used veterinary drugs, such as chloramphenicol, malachite green, leuco-malachite green and semi-carbazone, remains unproven.Therefore, in the present study, a rapid analytical method based on ultrasound-assisted SPE combined with colloidal gold test strips was developed and applied herein to determine the residues of these veterinary drugs in common aquatic products. Under optimized conditions, the method achieved a limit of detection in the range of 0.01–0.5 μg/kg, inter-day RSDs of the spiked samples in the ranges of 0.99–5.58 % and 1.6–7.2 %, and absolute recoveries in the range of 84.2–112.9 % using the colloidal gold immunoassay and enzyme-linked immunosorbent assay methods, respectively. The SPE column efficiently enriched the four veterinary drugs simultaneously, showing considerable potential for determining common veterinary drug residues in aquatic products.

中文翻译：

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新型固相萃取促进水产品中孔雀石绿、白色-孔雀石绿、氯霉素和氨基甲脲类代谢物的同步胶体金免疫层析分析


使用传统色谱柱材料的固相萃取 （SPE） 具有多项优势，但其同时提取和纯化常用兽药（如氯霉素、孔雀石绿、白色-孔雀石绿和半氨基甲酮）的能力仍未得到证实。因此，在本研究中，开发了一种基于超声辅助 SPE 结合胶体金试纸的快速分析方法，并应用于测定这些兽药在常见水产品中的残留。在优化条件下，使用胶体金免疫测定法和酶联免疫吸附测定方法，该方法的检测限为 0.01–0.5 μg/kg，加标样品的日间 RSD 为 0.99–5.58 % 和 1.6–7.2 %，绝对回收率为 84.2–112.9 %。SPE 色谱柱同时高效富集了 4 种兽药，在测定水产品中常见兽药残留方面显示出相当大的潜力。