The co-contamination of multiple mycotoxins in cereals brings serious food safety hazards. This work proposes a magnetic solid-phase extraction method based on polydopamine coated magnetic particles (Fe₃O₄@PDA), coupled with direct analysis in real time mass spectrometry (DART-MS), for high-throughput screening of 17 mycotoxins in six categories in rice, corn and wheat. The results suggest that Fe₃O₄@PDA is capable of co-extraction of all the mycotoxins within 3 min (adsorption rate above 87.3 %) and also exhibits commendable matrix purification ability, with matrix effects below −14.90 %, and recyclability, as it can be reused up to 5 times. DART-MS detection of all targets in a single sample can be completed within 20 s. The detection limits for mycotoxins ranged from 1.0 to 50.0 μg/kg, the recoveries were between 81.4 % and 117.5 %, and the relative standard deviations were less than 17.4 %, suggesting that the developed method had considerable sensitivity, accuracy, and precision.

中文翻译：

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谷物中多种典型霉菌毒素的快速高通量筛选


谷物中多种霉菌毒素的混合污染会带来严重的食品安全危害。本研究提出了一种基于聚多巴胺包被的磁性颗粒 （Fe₃O₄@PDA） 的磁性固相萃取方法，结合实时质谱直接分析 （DART-MS），用于高通量筛选水稻、玉米和小麦中 6 类 17 种霉菌毒素。结果表明，Fe₃O₄@PDA 能够在 3 分钟内共提取所有霉菌毒素（吸附率高于 87.3 %），并且还表现出值得称道的基质纯化能力，基质效应低于 −14.90 %，并且可回收性，因为它可以重复使用多达 5 次。可在 20 秒内完成单个样品中所有靶标的 DART-MS 检测。霉菌毒素的检测限范围为 1.0 至 50.0 μg/kg，回收率在 81.4% 至 117.5% 之间，相对标准偏差小于 17.4%，表明所开发的方法具有相当的灵敏度、准确度和精密度。