A novel two-stage carbon sequestration strategy (3 % and 10 % CO2) was developed and its feasibility was comprehensively demonstrated by multiple methods (pilot-scale cultivation, kinetics, economics and carbon fixation analysis). It was also a safe, efficient and low-cost harvesting strategy. At the end of the culture, astaxanthin production and content increased 2.3 and 2.2 times, respectively. Sedimentation rate (SR) was introduced for the first time to evaluate microalgae culture methods. The SR reached 82.2 % after 2 h of standing. Pilot-scale cultivation was achieved outdoors, with the optimal photobioreactor being a 40 L tubular photobioreactor (T-PBRs), which individually achieved 3.1 g/L and 2.3 % biomass and astaxanthin content. The maximum rate of carbon sequestration (227.9 mg/L/d) was observed in 40 L T-PBRs. The cost of producing 1 kg of astaxanthin-enriched Haematococcus pluvialis (H. pluvialis) was only 17.5 USD. This study brings new perspectives to carbon sequestration and the development of astaxanthin markets.

中文翻译：

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雨生红球藻的两阶段碳封存：从小规模到中试规模培养和数据质量监测的综合研究


开发了一种新的两阶段碳封存策略（3 % 和 10 % CO2），并通过多种方法（中试规模培养、动力学、经济性和碳固定分析）全面证明了其可行性。这也是一种安全、高效和低成本的收获策略。在培养结束时，虾青素产量和含量分别增加了 2.3 倍和 2.2 倍。首次引入沉降率 （SR） 来评估微藻培养方法。静置 2 小时后 SR 达到 82.2%。在户外实现了中试规模培养，最佳光生物反应器是 40 L 管式光生物反应器 （T-PBR），其单独达到 3.1 g/L 和 2.3% 的生物量和虾青素含量。在 40 L T-PBRs 中观察到最大碳封存速率 （227.9 mg/L/d）。生产 1 公斤富含虾青素的雨生红球藻 （H. pluvialis） 的成本仅为 17.5 美元。这项研究为碳封存和虾青素市场的发展带来了新的视角。