Curcuma aeruginosa, a valuable medicinal plant of Zingiberaceae family, has been used traditionally for the treatment of various diseases by the locals of Southeast Asian countries. The demand of this plant coupled with its slow growing habit have resulted in gradual declining of its population. The aim of the study is to establish an efficient micropropagation technique for production of C. aeruginosa seedlings as planting materials to produce the rhizomes to meet the market demand. When the rhizomatous buds were cultured for eight weeks on the gelled shoot proliferation medium, MS supplemented with 2 mg L−1 benzyladenine (BA) and 0.5 mg L−1 naphthalene acetic acid (NAA), only two to three shoots were produced from each explant. The newly established micropropagation technique by culturing the half-shoot explants in the aerated culture system for four weeks followed by another four weeks culture on gelled shoot proliferation medium, enabled an average of 15 shoots to produce from each explant. The in vitro seedlings derived via the conventional gelled medium had higher survival rate (96.7 ± 1.2 %) after acclimatization as compared to the established micropropagation technique with the aid of the aerated culture system (91.7 ± 1.7 %). This self-invented aerated culture system incorporate with gelled culture medium is an efficient mass propagation technique for C. aeruginosa. It enabled C. aeruginosa shoot explants to produce five times more clonal plantlets as compared to the conventional gelled medium culture.

中文翻译：

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使用自主研发的台式充气培养容器建立铜绿姜黄加速微繁殖系统


铜绿姜黄是姜科的一种有价值的药用植物，传统上被东南亚国家当地人用于治疗各种疾病。这种植物的需求加上其缓慢生长的习性导致其种群逐渐下降。本研究的目的是建立一种高效的微繁殖技术，用于生产铜绿梭菌幼苗作为种植材料来生产根茎以满足市场需求。当根茎芽在凝胶芽增殖培养基上培养 8 周时，MS 补充有 2 mg L-1 苄基腺嘌呤 （BA） 和 0.5 mg L-1 萘乙酸 （NAA），每个外植体仅产生 2 到 3 个芽。新建立的微繁殖技术通过在充气培养系统中培养半芽外植体 4 周，然后在凝胶芽增殖培养基上再培养 4 周，使每个外植体平均产生 15 个芽。与借助曝气培养系统建立的微繁殖技术 （91.7 ± 1.7 %） 相比，通过常规凝胶培养基衍生的体外幼苗在驯化后具有更高的存活率 （96.7 ± 1.2 %）。这种自行发明的充气培养系统与凝胶培养基相结合，是一种高效的铜绿假单胞菌大规模繁殖技术。与传统的凝胶培养基培养相比，它使铜绿假球杆菌芽外植体能够产生五倍的克隆植株。