Peach fruits are vulnerable to spoilage and easily infected by pathogens during postharvest storage. Fortunately, antagonistic yeast offers an effective method to control these postharvest diseases. In previous studies, we demonstrated the effectiveness of Wickerhamomyces anomalus in controlling postharvest peach diseases caused by Monilinia fructicola (brown rot), Aspergillus niger (aspergillosis) and Rhizopus stolonifer (soft rot), and the physiological control mechanisms were explored. However, the precise molecular response of peach fruit to W. anomalus-induced disease resistance is unknown. To address this knowledge gap, this study used RNA-seq technology to explore the underlying molecular mechanism of disease resistance induced by W. anomalus in peach fruit. Our investigation also examined the impact of W. anomalus on peach fruit's natural decay rate and storage quality. Treatment with W. anomalus significantly reduced the natural decay rate compared to the control group. Furthermore, W. anomalus treatment significantly increased the vitamin C content but did not affect the weight loss rate, hardness, titratable acid, soluble solid content and browning degree of peach fruits during storage. The transcriptome analysis of peach fruit treated with W. anomalus found that there were 1196 DEGs, of which 970 genes were up-regulated and 226 were down-regulated. Transcriptome analysis revealed that W. anomalus treatment triggered several signal transduction pathways in peach fruit, such as plant-pathogen interaction, MAPK signal transduction pathway, plant hormone signal transduction pathway, phenylpropane biosynthesis, glutathione metabolism. Differential regulation of these genes was associated with stress resistance, disease resistance, antioxidant capacity and production of antimicrobial compounds, ultimately enhancing the peach fruit's resistance to pathogens during storage.

中文翻译：

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分子网络参与提高 Wickerhamomyces anomalus 诱导的桃果实的抗病性


桃子果实在采后储存过程中容易变质，容易被病原体感染。幸运的是，拮抗酵母提供了一种有效的方法来控制这些采后疾病。在以前的研究中，我们证明了 Wickerhamomyces anomalus 在控制由 Monilinia fructicola （褐腐病）、黑曲霉 （曲霉病） 和 Rhizopus stolonifer （软腐病） 引起的桃后病害方面的有效性，并探讨了生理控制机制。然而，桃果实对 W. anomalus 诱导的抗病性的确切分子反应尚不清楚。为了解决这一知识空白，本研究使用 RNA-seq 技术探讨了 W. anomalus 诱导桃果实抗病的潜在分子机制。我们的调查还检查了 W. anomalus 对桃果实自然腐烂速率和储存质量的影响。与对照组相比，W. anomalus 治疗显著降低了自然腐烂率。此外，W. anomalus 处理显著提高了桃果实在贮藏过程中的维生素 C 含量，但不影响其失重率、硬度、可滴定酸、可溶性固形物含量和褐变度。对 W. anomalus 处理的桃果实进行转录组分析，发现有 1196 个 DEGs，其中 970 个基因上调，226 个基因下调。转录组分析显示，W. anomalus 处理触发了桃果实中的植物-病原体相互作用、MAPK 信号转导途径、植物激素信号转导途径、苯丙烷生物合成、谷胱甘肽代谢等多条信号转导途径。 这些基因的差异调控与抗逆性、抗病性、抗氧化能力和抗菌化合物的产生有关，最终增强了桃果实在贮藏过程中对病原菌的抵抗力。