The Nuclear Factor Y, Subunit A (NF-YA) gene family plays crucial roles in plant growth, development, and responses to abiotic stresses. In our study, we applied bioinformatics to identify NF-YA constituents in the tomato genome. We further analyzed their protein properties, evolutionary relationships, gene structures, chromosomal positions, collinearity, and expression patterns. Results revealed 10 NF-YA family genes in tomatoes. These genes encoding proteins ranging from 154 to 325 amino acids with four to five exons. Our analysis identified two segmental duplications events (SlNF-YA9/SlNF-YA7b and SlNF-YA10a/SlNF-YA10b) within the SlNF-YA cluster. Transcriptomic data showed high expression of SlNF-YA10a in roots, particularly induced under drought and saline-alkali conditions. Overexpressing or knockout of SlNF-YA10a found that SlNF-YA10a negatively regulated saline-alkali stress tolerance by influencing superoxide dismutase (SOD) and catalase (CAT) activities, ion homeostasis, and transcriptional levels of Na+/K+ transport-related genes. The research delves into the mechanisms that allow the tomato SlNF-YA10a gene to regulate tolerance to saline-alkali stress.

中文翻译：

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盐碱胁迫下番茄 SlNF-YA 基因的全基因组鉴定及 SlNF-YA10a 的功能表征


核因子 Y 亚基 A （NF-YA） 基因家族在植物生长、发育和对非生物胁迫的反应中起着至关重要的作用。在我们的研究中，我们应用生物信息学来鉴定番茄基因组中的 NF-YA 成分。我们进一步分析了它们的蛋白质特性、进化关系、基因结构、染色体位置、共线性和表达模式。结果显示番茄中存在 10 个 NF-YA 家族基因。这些基因编码的蛋白质范围从 154 到 325 个氨基酸不等，具有 4 到 5 个外显子。我们的分析在 SlNF-YA 集群中确定了两个节段重复事件 （SlNF-YA9/SlNF-YA7b 和 SlNF-YA10a/SlNF-YA10b）。转录组数据显示 SlNF-YA10a 在根中高表达，尤其是在干旱和盐碱条件下诱导。过表达或敲除 SlNF-YA10a 发现，SlNF-YA10a 通过影响超氧化物歧化酶 （SOD） 和过氧化氢酶 （CAT） 活性、离子稳态和 Na+/K+ 转运相关基因的转录水平，负向调节盐碱胁迫耐受性。该研究深入探讨了番茄 SlNF-YA10a 基因调节对盐碱胁迫耐受性的机制。