Chrysanthemum(Chrysanthemum × morifolium) branching is an important ornamental trait, which directly determines its ornamental quality and production. In this study, CmPP2C1 and CmPP2C2 were identified as interacting proteins of DgLsL and its function in branches was analyzed. Yeast two-hybrid assay (Y2H) and Luciferase complementation imaging assay (LCA) were used to demonstrate the interaction. The expression of CmPP2C1 and CmPP2C2 were significantly increased after decapitation. In addition, CmPP2C1 overexpression led to a significant decrease in endogenous abscisic acid (ABA) content and expression of related synthetic genes. This implies that CmPP2C1 and DgLsL may regulate chrysanthemum branching by participating in the ABA pathway. Taken together, our results revealed that protein phosphatases type 2C regulate branches by interacting with DgLsL and regulating Abscisic acid synthesis in Chrysanthemum × morifolium ‘Jinba’, which provides an important theoretical basis for the molecular breeding and production of the chrysanthemum.

中文翻译：

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2C 型蛋白磷酸酶通过与 DgLsL 相互作用并参与菊花×桑花中的脱落酸途径来调节分支


菊花（Chrysanthemum × morifolium）分枝是一种重要的观赏性状，直接决定其观赏品质和产量。本研究将 CmPP2C1 和 CmPP2C2 鉴定为 DgLsL 的互作蛋白，并分析其在分支中的功能。酵母双杂交测定 （Y2H） 和荧光素酶互补成像测定 （LCA） 用于证明相互作用。斩首后 CmPP2C1 和 CmPP2C2 的表达显著升高。此外，CmPP2C1 过表达导致内源性脱落酸 （ABA） 含量和相关合成基因的表达显著降低。这意味着 CmPP2C1 和 DgLsL 可能通过参与 ABA 途径来调节菊花分支。综上所述，我们的结果表明，2C 型蛋白磷酸酶通过与 DgLsL 相互作用和调节菊花 'Jinba' 中的脱落酸合成来调控分支×这为菊花的分子育种和生产提供了重要的理论基础。