Sources of resistance to sharka (Plum pox virus, PPV) have not been identified among peach [Prunus persica (L) Batsch] cultivars to date. However, several studies have shown the possibility of inducing resistance to PPV by grafting with the ‘Garrigues’ almond [P. dulcis (Mill) D. A. Webb] cultivar. The so-called “Garrigues effect” progressively reduces the symptoms of the disease and the accumulation of the virus in ‘GF305’ peach plants highly infected with PPV Dideron after grafting ‘Garrigues’. In addition, grafting 'Garrigues' onto ‘GF305’ before inoculation completely prevents infection by the virus. In this work, the ‘Garrigues’ cultivar has been used as an interstock to prevent PPV infection in peach. We have evaluated a collection of peach cultivars representing red and yellow skin, white and yellow flesh, and free and cling stones, in addition to platycarpa peaches (‘Maya’, ‘Transvalia’, ‘Romea’, ‘Catherine’, ‘Astoria’, ‘Tirrenia’, ‘Maruja M13’, ‘Levante 30′, ‘Alisio 15′ and ‘Carioca’). The cultivars were grafted onto ‘Garrigues’ that was already growing on ‘GF305’, ‘GF677’ and ‘Garnem’ rootstocks. Once the assayed plants were ready and fully developed [rootstock + interstock (‘Garrigues’) + peach cultivars], we graft inoculated the rootstocks and the growing peach plants with PPV at least three times. We were not able to obtain symptoms or detect the virus by RT-PCR in any of the cases, despite the proven susceptibility of these cultivars. In view of the results, we can affirm that using the ‘Garrigues’ graft as interstock could act as a kind of "vaccine" against PPV-Dideron in peach that is ready to be used commercially.

中文翻译：

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通过将 'Garrigues' 杏仁嫁接为间存木，在桃子中对李子痘病毒 （sharka） 的抗性


迄今为止，尚未在桃 [Prunus persica （L） Batsch] 品种中确定对 sharka（李痘病毒，PPV）的抗性来源。然而，几项研究表明，通过与 'Garrigues' 杏仁 [P. dulcis （Mill） DA Webb] 品种嫁接来诱导对 PPV 的抗性的可能性。所谓的“嘉理盖思效应”在嫁接“嘉理盖思”后逐渐减轻了疾病症状和病毒在高度感染 PPV Dideron 的“GF305”桃株中的积累。此外，在接种前将 'Garrigues' 嫁接到 'GF305' 上可以完全防止病毒感染。在这项工作中，'Garrigues' 栽培品种被用作隔苗以防止桃子感染 PPV。除了扁果桃（'Maya'、'Transvalia'、'Romea'、'Catherine'、'Astoria'、'Tirrenia'、'Maruja M13'、'Levante 30'、'Alisio 15' 和 'Carioca'），我们还评估了一系列代表红色和黄色皮肤、白色和黄色果肉、游离和粘附石头的桃子品种。这些品种被嫁接到已经生长在“GF305”、“GF677”和“Garnem”砧木上的“Garrigues”上。一旦化验的植物准备好并完全发育 [砧木 + 间木 （'Garrigues'） + 桃子品种]，我们用 PPV 嫁接接种砧木和生长中的桃子植物至少三次。尽管这些品种已被证明易感性，但我们在任何病例中都无法通过 RT-PCR 获得症状或检测到病毒。鉴于结果，我们可以肯定，使用 'Garrigues' 嫁接物作为库存可以作为一种针对桃子中 PPV-Dideron 的 “疫苗”，准备用于商业用途。