Benchtop NMR spectrometers, with moderate magnetic field strengths (B0=1−2.4T) and sub-ppm chemical shift resolution, are an affordable and portable alternative to standard laboratory NMR (B0≥7T). However, in moving to lower magnetic field instruments, sensitivity and chemical shift resolution are significantly reduced. The sensitivity limitation can be overcome by using hyperpolarisation to boost benchtop NMR signals by orders of magnitude. Of the wide range of hyperpolarisation methods currently available, dynamic nuclear polarisation (DNP), parahydrogen-induced polarisation (PHIP) and photochemically-induced dynamic nuclear polarisation (photo-CIDNP) have, to date, shown the most promise for integration with benchtop NMR for analytical applications. In this review we provide a summary of the theory of each of these techniques and discuss examples of how they have been integrated with benchtop NMR detection. Progress towards the use of hyperpolarised benchtop NMR for analytical applications, ranging from reaction monitoring to probing biomolecular interactions, is discussed, along with perspectives for the future.

中文翻译：

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用于分析应用的超极化台式 NMR 波谱


台式核磁共振波谱仪具有中等磁场强度 （B0=1−2.4T） 和亚 ppm 级化学位移分辨率，是标准实验室核磁共振 （B0≥7T） 的经济实惠且便携的替代品。然而，在转向低磁场仪器时，灵敏度和化学位移分辨率会显著降低。通过使用超极化将台式 NMR 信号提高几个数量级，可以克服灵敏度限制。在目前可用的各种超极化方法中，动态核极化 （DNP）、平行氢诱导极化 （PHIP） 和光化学诱导动态核极化 （photo-CIDNP） 迄今为止已显示出与台式 NMR 集成用于分析应用的最大前景。在这篇综述中，我们总结了这些技术的理论，并讨论了它们如何与台式 NMR 检测相结合的示例。讨论了将超极化台式 NMR 用于分析应用的进展，从反应监测到探测生物分子相互作用，以及未来的前景。