Dimethyldichlorosilane (DMDCS), an efficient silane coupling reagent appearing between the −OH groups of silica gel (SG) and picric acid, instantaneously produces a derivative enriched with nitro groups. The nitro group acting as an end-cap terminates the reaction and subsequently was converted into diazo to couple tyrosine’s phenol ring via its O-carbon, the inert center to immobilize horseradish peroxidase (HRP) in a multipoint mode. It maintains the status quo of the native enzyme’s protein folding and the entire protein groups’ chemistry. The molecular formula of the synthesized material was verified and appeared as {Si(OSi)₄ (H₂O)_x}_n{−O–Si(CH₃)₂–O–C₆H₂(N⁺≡N)₃(HRP)}₄·yH₂O; the parameters were evaluated as x = 0.5, n = 1158, and y = 752. The immobilized biocatalyst’s activity in organic solvents was 1.5 times better than that in an aqueous medium; it worked smoothly, wherein the activity in both solvents stabilized at six months and continued up to nine months at 63 ± 3% compared to the initial.

中文翻译：

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在富集重氮活化硅胶上固定化辣根过氧化物酶在有机溶剂中稳态下具有高生物催化性能


二甲基二氯硅烷 （DMDCS） 是一种出现在硅胶 （SG） 的 −OH 基团和苦味酸之间的高效硅烷偶联试剂，可瞬间产生富含硝基的衍生物。作为端盖的硝基终止反应，随后转化为重氮，通过其 O-carbon（惰性中心）以多点模式固定辣根过氧化物酶 （HRP） 的惰性中心）偶联酪氨酸的酚环。它维持天然酶的蛋白质折叠和整个蛋白质组化学的现状。对合成材料的分子式进行了验证，表现为{Si（OSi）₄ （H₂O）_x}_n{−O–Si（CH₃）₂–O–C₆H₂（N⁺≡N）₃（HRP）}₄·yH₂O;参数评估为 x = 0.5 、 n = 1158 和 y = 752。固定化生物催化剂在有机溶剂中的活性比在水性介质中的活性高 1.5 倍;它运行顺利，其中两种溶剂中的活性稳定在 6 个月时，并与初始溶剂相比持续到 63% ± 3% 长达 9 个月。