The important constituents of rosemary herb extract are carnosic acid (CarA), carnosol (Car) and rosmanol (Ros), which are burdensome to the human body when consumed in excessive amounts. Using a computer-assisted method, we prepared 3B10-antibody with both broad-spectrum and high affinity for CarA, Car and Ros with median inhibitory concentration of 0.41 ng/mL, 0.45 ng/mL, and 0.53 ng/mL, respectively. Subsequently, a quantitative lateral flow immunoassay (LFIA) was developed to identify CarA, Car and Ros in ham sausage and dairy within 30 min, and the respectively calculated limit of detection values of 0.044 μg/kg, 0.148 μg/kg and 0.169 μg/kg in sample, which could meet maximum additive limits. The quantitative results of LFIA method in positive samples were consistent with that of the instrumental method.These results also show that the high recognition performance and reliability of the LFIA strips validate the field test method.

中文翻译：

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用于快速检测火腿、香肠和乳制品中迷迭香草本提取物的定量免疫层析法


迷迭香草本提取物的重要成分是鼠尾草酸 （CarA）、鼠尾草醇 （Car） 和迷迭香醇 （Ros），过量食用会对人体造成负担。使用计算机辅助方法，我们制备了对 CarA、Car 和 Ros 具有广谱和高亲和力的 3B10 抗体，中位抑制浓度分别为 0.41 ng/mL、0.45 ng/mL 和 0.53 ng/mL。随后，建立定量侧流免疫分析法 （LFIA），在 30 min 内鉴定火腿肠和乳制品中的 CarA、Car 和 Ros，样品中分别计算出 0.044 μg/kg、0.148 μg/kg 和 0.169 μg/kg 的检测限，可满足最大添加剂限量。LFIA 法在阳性样品中的定量结果与仪器法一致。这些结果还表明，LFIA 试纸条的高识别性能和可靠性验证了现场测试方法的有效性。