Yarrowia lipolytica, an oleaginous yeast, shows promise for industrial fermentation due to its robust acetyl-CoA flux and well-developed genetic engineering tools. However, its lack of an active xylose metabolism restricts the conversion of cellulosic sugars to valuable products. To address this, metabolic engineering, and adaptive laboratory evolution (ALE) were applied to the Y. lipolytica PO1f strain, resulting in an efficient xylose-assimilating strain (XEV). Whole-genome sequencing (WGS) of the XEV followed by reverse engineering revealed that the amplification of the heterologous oxidoreductase pathway and a mutation in the GTPase-activating protein gene (YALI0B12100g) might be the primary reasons for improved xylose assimilation in the XEV strain. When a sorghum hydrolysate was used, the XEV strain showed superior xylose consumption and lipid production compared to its parental strain (X123). This study advances our understanding of xylose metabolism in Y. lipolytica and proposes effective metabolic engineering strategies for optimizing lignocellulosic hydrolysates.

中文翻译：

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以木质纤维素水解物生产脂质的 Yarrowia lipolytica 的工程与进化


Yarrowia lipolytica 是一种含油酵母，由于其强大的乙酰辅酶 A 通量和发达的基因工程工具，显示出工业发酵的前景。然而，它缺乏活跃的木糖代谢限制了纤维素糖转化为有价值的产品。为了解决这个问题，将代谢工程和适应性实验室进化 （ALE） 应用于 Y. lipolytica PO1f 菌株，从而产生高效的木糖同化菌株 （XEV）。XEV 的全基因组测序 （WGS） 后进行逆向工程显示，异源氧化还原酶通路的扩增和 GTP 酶激活蛋白基因 （YALI0B12100g） 的突变可能是 XEV 菌株中木糖同化改善的主要原因。当使用高粱水解物时，XEV 菌株与其亲本菌株 （X123） 相比表现出优异的木糖消耗和脂质产生。这项研究促进了我们对 Y. lipolytica 中木糖代谢的理解，并提出了优化木质纤维素水解物的有效代谢工程策略。