当前位置:
X-MOL 学术
›
Biochem. J.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Mitigating Methylglyoxal-Induced Glycation Stress: The Protective Role of Iron, Copper, and Manganese Coordination Compounds in Saccharomyces cerevisiae.
Biochemical Journal ( IF 4.4 ) Pub Date : 2024-11-13 , DOI: 10.1042/bcj20240390 Maria Eduarda Sant'Ana Faria do Espírito Santo,Bárbara Filgueiras Frascino,Larissa M M Mattos,Daniele Cabral Pires,Simone S C de Oliveira,Lucas B Menezes,Bernardo Ferreira Braz,Ricardo Erthal Santelli,André Santos,Adolfo Horn Junior,Christiane Fernandes,Marcos Pereira
Biochemical Journal ( IF 4.4 ) Pub Date : 2024-11-13 , DOI: 10.1042/bcj20240390 Maria Eduarda Sant'Ana Faria do Espírito Santo,Bárbara Filgueiras Frascino,Larissa M M Mattos,Daniele Cabral Pires,Simone S C de Oliveira,Lucas B Menezes,Bernardo Ferreira Braz,Ricardo Erthal Santelli,André Santos,Adolfo Horn Junior,Christiane Fernandes,Marcos Pereira
Glycation-induced stress (G-iS) is a physiological phenomenon that leads to the formation of advanced glycation end-products (AGEs), triggering detrimental effects such as oxidative stress, inflammation, and damage to intracellular structures, tissues, and organs. This process is particularly relevant because it has been associated with various human pathologies, including cancer, neurodegenerative diseases, and diabetes. As therapeutic alternatives, coordination compounds with antioxidant activity show promising potential due to their versatility in attenuating oxidative stress and inflammation. Herein, we investigated the antioxidant-related protective potential of a series of complexes: [Cu(II)(BMPA)Cl2] (1), [Fe(III)(BMPA)Cl3] (2), and [Cl(BMPA)MnII-(μ-Cl)2-MnII(BMPA)-(μ-Cl)- MnII(BMPA)(Cl)2]•5H2O (3), all synthesized with the ligand bis-(2-pyridylmethyl)amine (BMPA) in Saccharomyces cerevisiae exposed to G-iS caused by methylglyoxal (MG). Pre- treatment with complexes 1 - 3 proved highly effective, increasing yeast tolerance to G-iS and attenuating mitochondrial dysfunction. This observed phenotype appears to result from a reduction in intracellular oxidation, lipid peroxidation levels, and glycation. Additionally, an increase in the activity of the antioxidant enzymes superoxide dismutase and catalase was observed following treatment with complexes 1 - 3. Notably, although complexes 1 - 3 provided significant protection against oxidative stress induced by H2O2 and menadione, their protective role was more effective against MG-induced glycation stress. Our results indicate that these complexes possess both antiglycation and antioxidant properties, warranting further investigation as potential interventions for mitigating glycation and oxidative stress-related pathologies.
中文翻译:
减轻甲基乙二醛诱导的糖基化应激:铁、铜和锰配位化合物在酿酒酵母中的保护作用。
糖基化诱导应激 (G-iS) 是一种生理现象,会导致晚期糖基化终末产物 (AGEs) 的形成,引发氧化应激、炎症等有害影响,并对细胞内结构、组织和器官造成损害。这个过程特别相关,因为它与各种人类病理有关,包括癌症、神经退行性疾病和糖尿病。作为治疗替代品,具有抗氧化活性的配位化合物因其在减轻氧化应激和炎症方面的多功能性而显示出有前途的潜力。在此,我们研究了一系列复合物的抗氧化相关保护潜力:[Cu(II)(BMPA)Cl2] (1)、[Fe(III)(BMPA)Cl3] (2) 和 [Cl(BMPA)MnII-(μ-Cl)2-MnII(BMPA)-(μ-Cl)-MnII(BMPA)(Cl)2]•5H2O (3),均由甲基乙二醛 (MG) 引起的酿酒酵母中的配体双-(2-吡啶甲基)胺 (BMPA) 合成。用复合物 1 - 3 进行预处理证明非常有效,增加了酵母对 G-iS 的耐受性并减轻了线粒体功能障碍。这种观察到的表型似乎是由于细胞内氧化、脂质过氧化水平和糖基化降低的结果。此外,在用复合物 1 - 3 处理后观察到抗氧化酶超氧化物歧化酶和过氧化氢酶的活性增加。值得注意的是,尽管复合物 1 - 3 对 H 2 O 2 和甲萘醌诱导的氧化应激提供了显著的保护作用,但它们对 MG 诱导的糖基化应激的保护作用更有效。 我们的结果表明,这些复合物具有抗糖化和抗氧化特性,值得进一步研究作为减轻糖基化和氧化应激相关病理的潜在干预措施。
更新日期:2024-11-13
中文翻译:
减轻甲基乙二醛诱导的糖基化应激:铁、铜和锰配位化合物在酿酒酵母中的保护作用。
糖基化诱导应激 (G-iS) 是一种生理现象,会导致晚期糖基化终末产物 (AGEs) 的形成,引发氧化应激、炎症等有害影响,并对细胞内结构、组织和器官造成损害。这个过程特别相关,因为它与各种人类病理有关,包括癌症、神经退行性疾病和糖尿病。作为治疗替代品,具有抗氧化活性的配位化合物因其在减轻氧化应激和炎症方面的多功能性而显示出有前途的潜力。在此,我们研究了一系列复合物的抗氧化相关保护潜力:[Cu(II)(BMPA)Cl2] (1)、[Fe(III)(BMPA)Cl3] (2) 和 [Cl(BMPA)MnII-(μ-Cl)2-MnII(BMPA)-(μ-Cl)-MnII(BMPA)(Cl)2]•5H2O (3),均由甲基乙二醛 (MG) 引起的酿酒酵母中的配体双-(2-吡啶甲基)胺 (BMPA) 合成。用复合物 1 - 3 进行预处理证明非常有效,增加了酵母对 G-iS 的耐受性并减轻了线粒体功能障碍。这种观察到的表型似乎是由于细胞内氧化、脂质过氧化水平和糖基化降低的结果。此外,在用复合物 1 - 3 处理后观察到抗氧化酶超氧化物歧化酶和过氧化氢酶的活性增加。值得注意的是,尽管复合物 1 - 3 对 H 2 O 2 和甲萘醌诱导的氧化应激提供了显著的保护作用,但它们对 MG 诱导的糖基化应激的保护作用更有效。 我们的结果表明,这些复合物具有抗糖化和抗氧化特性,值得进一步研究作为减轻糖基化和氧化应激相关病理的潜在干预措施。