The high level of fluoride in Qingzhuan tea (QZT) poses a potential health risk to consumers. This study aims to explore the binding behavior of purified Qingzhuan tea polysaccharides (pTPS) and fluoride ions (F^–), as well as their regulatory role in the digestion and metabolism of fluoride. The sugar content of pTPS was 94.64 ± 3.01%, with a molecular weight of 7.373 × 10⁴ Da and high homogeneity. The effects of different proportions and environmental conditions on the adsorption of F^– by pTPS were investigated. The influence of the complexation of pTPS and F^– on the digestion and metabolism of fluoride was explored using an in vitro gastrointestinal digestion model and C57BL/6 mice. The structural alterations of pTPS were observed during simulated gastrointestinal digestion. Furthermore, pTPS were found to reduce serum fluoride levels and inhibit accumulation in major organs and tissues, especially the heart, liver, kidneys, muscles, and femur. This study investigated the binding pattern between fluorine and pTPS and its influence on the digestion and absorption of fluorine, providing a promising potential for pTPS as a bioadsorbent of fluorine to alleviate the toxicity of fluorine in QZT, which laid a theoretical foundation for the safety of consumption of QZT.

中文翻译：

---

青转茶多糖对 F– 吸附的影响：分子结构、结合行为以及体外和体内消化和代谢


青烧茶 （QZT） 中的高含量氟化物对消费者构成潜在的健康风险。本研究旨在探讨纯化青转茶多糖 （pTPS） 与氟离子 （F^–） 的结合行为，以及它们在氟化物消化代谢中的调节作用。pTPS 的糖含量为 94.64 ± 3.01%，分子量为 7.373 × 10⁴ Da，均一性高。研究了不同比例和环境条件对 pTPS 吸附 F^– 的影响。使用体外胃肠道消化模型和 C57BL/6 小鼠探讨 pTPS 和 F^– 络合对氟化物消化和代谢的影响。在模拟胃肠道消化过程中观察到 pTPS 的结构改变。此外，发现 pTPS 可降低血清氟化物水平并抑制主要器官和组织中的积累，尤其是心脏、肝脏、肾脏、肌肉和股骨。本研究探讨了氟与 pTPS 的结合模式及其对氟消化吸收的影响，为 pTPS 作为氟的生物吸附剂减轻 QZT 中氟的毒性提供了有前途的潜力，为QZT 的食用安全性奠定了理论基础。