Tomato yellow leaf curl virus disease has seriously threatened the quality and yield of tomatoes. In this study, we investigated the role of amiRNA technology in disease resistance in tomatoes (cherry tomato and large-fruited tomato) and analyzed the physiological and molecular mechanisms of disease resistance in transgenic plants. TYLCV contains six functional genes, of which the C1, C2, and V1 genes have more phosphorylation sites and glycosylation sites, and the protein structure is more complex. The virus replication was inhibited, the peroxidation of membrane lipids was reduced, and disease resistance was enhanced in all transgenic cherry tomato (J6) plants in which the C1, C2, and V1 genes were silenced, respectively. Similarly, silencing of the C1 gene enhanced disease resistance in large-fruited tomatoes. In conclusion, amiRNA technology hinders viral replication, leading to reduced activity of the tomato plant-pathogen interaction pathway and weakening tomato-virus interactions, thereby improving disease resistance.

中文翻译：

---

AmiRNA 技术通过抑制 TYLCV 复制抑制植物-病原体相互作用途径，增强番茄病害抗性


番茄黄卷叶病毒病严重威胁了番茄的品质和产量。在本研究中，我们研究了 amiRNA 技术在番茄 （樱桃番茄和大果实番茄） 抗病性中的作用，并分析了转基因植物抗病性的生理和分子机制。TYLCV 包含 6 个功能基因，其中 C1、C2 和 V1 基因具有更多的磷酸化位点和糖基化位点，蛋白质结构更复杂。C1、C2 和 V1 基因分别沉默的所有转基因樱桃番茄 （J6） 植株的病毒复制受到抑制，膜脂质的过氧化减少，抗病性增强。同样，C1 基因的沉默增强了大果实番茄的抗病性。总之，amiRNA 技术阻碍病毒复制，导致番茄植物-病原体相互作用途径活性降低，削弱番茄-病毒相互作用，从而提高抗病性。