As the concept of precision nutrition has been gradually popularized in recent years, the relationship between the structure of starch–polyphenol complexes with significant health effects and their nutritional functions has been progressively investigated. In this study, G50 high-amylose maize starch with different molecular weights was first prepared by pullulanase and α-amylase, and their effects on the structural formation, digestion properties, and release behaviors of the starch–resveratrol (RA) complex were discussed. The results confirmed that enzyme-treated starch could enhance intermolecular hydrogen bonding and hydrophobic interactions between starch and RA in a high-pressure homogeneous (HPH) environment, forming stable single-helix and V-type crystalline structures while reducing the B-type crystalline structures. Meanwhile, the in vitro experiment showed that when the RA addition was 3%, the resistant starch content of the starch–RA complex could reach 60.3%, and its RA colonic transport rate could reach more than 97%. Interestingly, the starch–RA complex with a relatively higher V-type crystalline structure content contributed to the production of short-chain fatty acids (SCFAs), especially butyrate, and it might be effective in carbohydrate metabolism and immunometabolism by promoting the functions of Phascolarctobacteriu and Alistipes. These findings provide new ideas for the design of the nutritional functions of RS.

中文翻译：

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通过酶处理控制淀粉分子量有助于在高压均质环境中形成 V 型淀粉-白藜芦醇复合物及其对肠道微生物群的调节作用


近年来，随着精准营养概念的逐渐普及，具有显著健康影响的淀粉-多酚复合物的结构与其营养功能之间的关系也逐渐得到研究。本研究首先用支链淀粉酶和 α-淀粉酶制备了不同分子量的 G50 高直链玉米淀粉，并讨论了它们对淀粉-白藜芦醇 （RA） 复合物的结构形成、消化特性和释放行为的影响。结果证实，在高压均相 （HPH） 环境中，酶处理淀粉可以增强分子间氢键和淀粉与 RA 之间的疏水相互作用，形成稳定的单螺旋和 V 型晶体结构，同时减少 B 型晶体结构。同时，体外实验表明，当 RA 添加量为 3% 时，淀粉-RA 复合物的抗性淀粉含量可达 60.3%，其 RA 结肠转运率可达 97% 以上。有趣的是，具有相对较高的 V 型晶体结构含量的淀粉-RA 复合物有助于短链脂肪酸 （SCFA） 的产生，尤其是丁酸盐，它可能通过促进 Phascolarctobacteriu 和 Alistipes 的功能对碳水化合物代谢和免疫代谢有效。这些发现为 RS 营养功能的设计提供了新思路。