Reaction-based fluorogenic sensing of lethal cyanide anions in aqueous matrices remains a big challenge. We have revisited the reported approach about an intramolecular crossed-benzoin reaction leading to the release of a phenol-based fluorophore. Fluorescence assays and RP-HPLC-MS analyses have helped us to highlight its limitations related to poor aqueous stability of probes and impossibility to achieve molecular amplification despite the assumed catalytic activation mechanism. Traceless cleavable linker strategies were considered to obtain usable cyanide-responsive chemodosimeters and statistical analyses of fluorescence data have been conducted in depth to accurately delineate their sensing performances, especially the limit of detection (LOD).

中文翻译：

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通过分子内交联安息香反应对纯水性介质中的氰离子进行荧光检测：揭露的局限性和可能的解决方案


对水性基质中的致命氰化物阴离子进行基于反应的荧光传感仍然是一个很大的挑战。我们重新审视了关于分子内交联安息香反应导致苯酚基荧光团释放的报道方法。荧光分析和 RP-HPLC-MS 分析帮助我们突出了其局限性，这些局限性与探针的水稳定性差以及尽管假设了催化活化机制但仍无法实现分子扩增有关。考虑使用无痕可切割接头策略来获得可用的氰化物响应性化学剂量计，并对荧光数据进行了深入统计分析，以准确描述其传感性能，尤其是检测限 （LOD）。