Hydrogel microspheres with monodisperse and homogeneous dimensions have potential application in the field of three-dimensional (3D) cell culture due to its ability to provide a similar microenvironment. Currently, alginate hydrogel microspheres (AHMs) have received much attention due to the favorable properties of alginate such as biocompatibility, inexpensiveness, nontoxicity, and biodegradability. The fabrication methods of AHMs mainly include extrusion, electrostatic dripping and microfluidic chip techniques. These current methods suffer trade-offs between operational complexity, fabrication cost and practical application.

中文翻译：

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基于多孔板的多功能平台，用于在线制备藻酸盐水凝胶微球和原位 3D 细胞培养


具有单分散和均质尺寸的水凝胶微球由于能够提供类似的微环境，因此在三维 （3D） 细胞培养领域具有潜在的应用。目前，海藻酸盐水凝胶微球 （AHM） 因其海藻酸盐的生物相容性、廉价、无毒和生物降解性等良好特性而受到广泛关注。AHMs的制造方法主要包括挤出、静电滴落和微流控芯片技术。这些当前方法在操作复杂性、制造成本和实际应用之间进行权衡。