Gene therapy has emerged as a promising approach to address challenging cardiovascular diseases. Extensive efforts have been focused on developing highly efficient gene vectors with precise delivery techniques to enhance its effectiveness. In this study, we present multifunctional dopamine-gelatin microneedle patches with gene therapy capabilities to achieve perivascular gene delivery for intimal hyperplasia treatment. These patches that were fabricated through freeze-drying of gelatin are with recombinant adeno-associated virus (rAAVs)-carrying tips and dopamine coating backing layers. The lyophilized gelatin could not only effectively preserve the therapeutic activity of rAAVs but could also demonstrate the capability to penetrate the adventitia for efficient delivery. The incorporation of dopamine facilitated patch adhesion and extended the release duration. Based on these advantages, we have demonstrated that the rAAVs-loaded microneedle patches (AMNPs) behave satisfactorily in perivascular gene delivery to inhibit carotid artery restenosis in rats. These features indicate that the AMNPs are clinically valuable in the treatment of vascular intimal hyperplasia diseases.

中文翻译：

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用于血管周围基因递送和血管内膜增生治疗的多功能微针贴片


基因疗法已成为解决具有挑战性的心血管疾病的一种有前途的方法。人们广泛致力于开发具有精确递送技术的高效基因载体，以提高其有效性。在这项研究中，我们提出了具有基因治疗能力的多功能多巴胺-明胶微针贴剂，以实现血管周围基因递送以治疗内膜增生。这些通过明胶冷冻干燥制成的贴剂带有携带重组腺相关病毒 （rAAV） 的尖端和多巴胺涂层背衬层。冻干明胶不仅可以有效保持 rAAV 的治疗活性，还可以证明能够穿透外膜进行有效递送。多巴胺的掺入促进了贴剂粘附并延长了释放持续时间。基于这些优势，我们已经证明负载 rAAVs 的微针贴片 （AMNPs） 在血管周围基因递送中表现令人满意，以抑制大鼠颈动脉再狭窄。这些特征表明 AMNPs 在治疗血管性内膜增生疾病方面具有临床价值。