Summary Here, we investigated the molecular genetic basis of mite domatia, structures on the underside of leaves that house mutualistic mites, and intraspecific variation in domatia size in Vitis riparia (riverbank grape). Domatia and leaf traits were measured, and the transcriptomes of mite domatia from two genotypes of V. riparia with distinct domatia sizes were sequenced to investigate the molecular genetic pathways that regulate domatia development and intraspecific variation in domatia traits. Key trichome regulators as well as auxin and jasmonic acid are involved in domatia development. Genes involved in cell wall biosynthesis, biotic interactions, and molecule transport/metabolism are upregulated in domatia, consistent with their role in domatia development and function. This work is one of the first to date that provides insight into the molecular genetic bases of mite domatia. We identified key genetic pathways involved in domatia development and function, and uncovered unexpected pathways that provide an avenue for future investigation. We also found that intraspecific variation in domatia size in V. riparia seems to be driven by differences in overall leaf development between genotypes.

中文翻译：

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小而螨：使用转录组学研究 Vitis riparia 中螨虫 domatia 发育和种内变异的分子遗传基础


摘要 在这里，我们研究了螨虫的分子遗传基础、容纳共生螨虫的叶子下侧的结构以及 Vitis riparia（河岸葡萄）中 domatia 大小的种内变化。测量 domatia 和叶片性状，并对来自不同 domatia 大小的 V. riparia 的两种基因型的螨 domatia 转录组进行测序，以研究调节 domatia 发育和 domatia 性状种内变异的分子遗传途径。关键的毛状体调节因子以及生长素和茉莉酸参与 domatia 的发育。参与细胞壁生物合成、生物相互作用和分子运输/代谢的基因在 domatia 中上调，这与它们在 domatia 发育和功能中的作用一致。这项工作是迄今为止最早提供对螨虫 domatia 分子遗传基础的见解的工作之一。我们确定了参与 domatia 发育和功能的关键遗传途径，并发现了为未来研究提供途径的意想不到的途径。我们还发现，V. riparia 中 domatia 大小的种内变化似乎是由基因型之间整体叶片发育的差异驱动的。