Tribulus terrestris L. is a herb renowned for its abundance of saponins, flavonoids, and alkaloids, which are utilized in treating various health conditions. Protodioscin functions by enhancing the conversion of testosterone into potent dihydrotestosterone, stimulating an increase in libido, red blood cell production from the bone marrow, and muscle development. Contemporary ultrasound-assisted-extraction (UAE) process employing green extraction solvents was selected to design the required separation system. The experimental plan was developed based on the independent operating variables – extraction time, operating temperature and solvent system composition in order to determine the influence of defined parameters and their interactions on the extraction yield and the presence of protodioscin. The 3D-RSM approach was introduced to determine the optimal values of studied independent variables in the area of maximal extraction yield. UAE process performed at optimal operating conditions generated maximal extraction yield (31 %, w/w) and protodioscin content of 5.9 mg/g dry plant matrix. Experimental data was used to develop an ANN for the defined extraction system using the operating variables values as the input matrix and observed yield as target matrix. Successfully designed and trained ANN generated high correlation (r = 0.9992) between observed data and predictive model’ outputs, and MSE value of 0.29107.

中文翻译：

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超声辅助提取富含土蒺藜 L 的原薯荣皂苷提取物的 ANN 建模和 RSM 优化。


Tribulus terrestris L. 是一种草药，以其丰富的皂苷、类黄酮和生物碱而闻名，用于治疗各种健康状况。原薯蓣皂苷通过增强睾酮转化为有效的二氢睾酮、刺激增加、骨髓红细胞生成和肌肉发育来发挥作用。选择采用绿色萃取溶剂的现代超声辅助萃取 （UAE） 工艺来设计所需的分离系统。实验计划是根据独立操作变量（提取时间、工作温度和溶剂系统组成）制定的，以确定定义参数及其相互作用对提取产量和原薯蓣皂苷存在的影响。引入 3D-RSM 方法以确定所研究的自变量在最大提取产量区域的最佳值。在最佳操作条件下进行的 UAE 工艺产生了最大的提取率 （31 %，w/w） 和 5.9 mg/g 干燥植物基质的原薯蓣星含量。使用实验数据为定义的提取系统开发 ANN，使用操作变量值作为输入矩阵，观察产量作为目标矩阵。成功设计和训练的 ANN 在观察到的数据和预测模型的输出之间产生了高度相关性 （r = 0.9992），MSE 值为 0.29107。