This study presents the synthesis and optimization of Methylene polyethyl glycol −Polystyrene (mPEG-PS) Diblock (DIP) copolymer-based solid lipid nanoparticles (SLNs) using ultrasonication for advanced drug delivery systems targeting the human immunodeficiency virus (HIV-1). The mPEG-PS block copolymer was synthesized by ring opening polymerization mechanism under nitrogen atmosphere for 24hrs and characterized using Fourier Transform Infrared Spectroscopy (FTIR) spectroscopy and NMR, confirming the formation of DIP polymers. Optimization of SLNs formulation was achieved through a systematic approach, utilizing response surface methodology, optimal conditions for SLNs synthesis were determined, resulting in nanoparticles with a particle size of 198 nm and an entrapment efficiency of 67.42 %. Cell viability assays, quantitative PCR for viral DNA analysis, caspase-3 enzyme assays, and quantitative uptake studies using High Performance Liquid Chromatography (HPLC) provided quantitative insights into the efficacy and biocompatibility of the synthesized nanoparticles. The experimental data demonstrate that nanoparticle treatments significantly influence cellular responses, providing valuable insights into their therapeutic potential and underlying mechanisms. By employing precise experimental methods alongside rigorous analytical techniques, this study enhances our understanding of nanoparticle-based drug delivery systems, particularly in the context of HIV treatment. These findings pave the way for optimizing therapeutic strategies to improve patient outcomes.

中文翻译：

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超声介导的基于二嵌段聚合物的纳米颗粒合成用于高级药物递送系统：洞察和优化


本研究介绍了使用超声处理合成和优化基于亚甲基聚乙二醇-聚苯乙烯 （mPEG-PS） 二嵌段 （DIP） 共聚物的固体脂质纳米颗粒 （SLN），用于针对人类免疫缺陷病毒 （HIV-1） 的先进药物递送系统。在氮气气氛下通过开环聚合机制合成 mPEG-PS 嵌段共聚物 24 小时，并使用傅里叶变换红外光谱 （FTIR） 光谱和 NMR 进行表征，证实了 DIP 聚合物的形成。通过系统方法实现 SLNs 配方的优化，利用响应面方法，确定了 SLNs 合成的最佳条件，得到粒径为 198 nm、包封效率为 67.42 % 的纳米颗粒。细胞活力测定、用于病毒 DNA 分析的定量 PCR、caspase-3 酶测定以及使用高效液相色谱 （HPLC） 的定量摄取研究为合成纳米颗粒的功效和生物相容性提供了定量见解。实验数据表明，纳米颗粒处理显着影响细胞反应，为了解其治疗潜力和潜在机制提供了有价值的见解。通过采用精确的实验方法和严格的分析技术，这项研究增强了我们对基于纳米颗粒的药物递送系统的理解，尤其是在 HIV 治疗的背景下。这些发现为优化治疗策略以改善患者预后铺平了道路。