Agar production is accompanied by a large amount of wastewater, which threatens the ecological environment and wastes biomass resources. The high-value utilization of biomass resources in wastewater is one of the key factors in wastewater treatment. We investigated the conversion process and antifungal mechanism of large molecule polysaccharides in wastewater into small molecule substances with antifungal activity through anaerobic fermentation. The results indicated that anaerobic fermentation of GAW achieved inhibition rates of 91.06 % and 88.94 % against Alternaria alternata and Alternaria spp. 16S rDNA sequencing and metabolomics revealed that dominant species such as Blautia, Agathobacter and Sphingomonas converted polysaccharide into phenolic acids like procyanidin C and columbidin. These substances disrupted the integrity of fungal cells, leading to their death. The preparation of composite antifungal agents using fermentation products effectively inhibited cherry tomato spoilage and toxin production. This study provided reliable technical support for the reuse of seaweed waste resources.

中文翻译：

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Gracilaria 琼脂废水厌氧发酵后植物病原真菌的防霉机理及应用


琼脂生产伴随着大量的废水，威胁着生态环境，浪费了生物质资源。废水中生物质资源的高值化利用是废水处理的关键因素之一。我们研究了废水中大分子多糖通过厌氧发酵转化为具有抗真菌活性的小分子物质的过程和抗真菌机制。结果表明，GAW 厌氧发酵对链格孢菌和链格孢菌属 16S 的抑制率分别为 91.06 % 和 88.94 %。rDNA 测序和代谢组学显示，Blautia、Agathobacter 和 Sphingomonas 等优势物种将多糖转化为酚酸，如原花青素 C 和氨酰胺。这些物质破坏了真菌细胞的完整性，导致它们死亡。利用发酵产物制备复合防霉剂可有效抑制樱桃番茄腐败和毒素产生。本研究为海藻废弃物资源的再利用提供了可靠的技术支持。