The rapid development of high-productivity strains is fundamental for bio-manufacture in industry. Here, Multi-module metabolic engineering was implemented to reprogram Escherichia coli, enabling it to rapidly transitioning from zero-producer to hyperproducer of L-threonine. Firstly, the synthesis pathway of L-threonine was rationally divided into five modules, and the rapid production of L-threonine was achieved by optimizing the expression of genes in each module. Subsequently, the capture and fixation of CO2 was enhanced to improve L-threonine yield. Dynamically balancing cell growth and yield by quorum-sensing system resulted in the accumulation of L-threonine up to 34.24 g/L. Ultimately, the THR36-L19 strain accumulated 120.1 g/L L-threonine with 0.425 g/g glucose in a 5 L bioreactor. This is the highest yield for de novo producing L-threonine reported to date and without the use of exogenous inducers and antibiotics in the fermentation process. It also provided an effective technological guidence for the zero-to-overproduction of other chemicals.

中文翻译：

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多模块工程指导高效生产 L-苏氨酸的细胞工厂的开发


高生产率菌株的快速发展是工业生物制造的基础。在这里，实施了多模块代谢工程来重编程大肠杆菌，使其能够从 L-苏氨酸的零生产者快速过渡到超生产者。首先，将 L-苏氨酸的合成途径合理划分为 5 个模块，通过优化每个模块中基因的表达来实现 L-苏氨酸的快速生成。随后，增强了 CO2 的捕获和固定，以提高 L-苏氨酸产量。通过群体感应系统动态平衡细胞生长和产量导致 L-苏氨酸积累高达 34.24 g/L。最终，THR36-L19 菌株在 5 L 生物反应器中积累了 120.1 g/L L-苏氨酸和 0.425 g/g 葡萄糖。这是迄今为止报道的从头生产 L-苏氨酸的最高产量，并且在发酵过程中不使用外源诱导剂和抗生素。它还为其他化学品的零生产或超生产提供了有效的技术指导。