To assess the inherent effects of light–dark cycle on the aniline degradation and nitrogen removal in algal-bacterial symbiotic system, three groups with different photoperiods (0L:12D;6L:6D;12L:0D) were set up. The results revealed that the aniline degradation rate of the three systems all surpassed 99 %, the total nitrogen removal rate of Z2-6L:6D was approximately 36 % higher than Z1-0L:12D eventually, the Z1-0L:12D was restrained by NH4+-N assimilation and nitrification while anoxic denitrification in Z3-12L:0D. The disappearance of microalgae biomass was accompanied by the sharp decreased of polysaccharide in Z1 and longer illumination suppressed the secretion of extracellular polymeric substances, the Z3 yielded slightly superior biomass production despite the double illumination compared with Z2. Moreover, high throughput sequencing analysis illustrated that the microbial community structure in Z2 was more abundant and even than Z3, the TM7a, norank_f__norank_o__Saccharimonadales, Ellin6067 and Scenedesmus proliferated wildly and the photoinhibition to functional genus was effectively alleviated in Z2.

中文翻译：

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利用昼夜动力学：了解苯胺胁迫下明暗循环对藻类-细菌共生系统的影响


为了评估光暗循环对藻-细菌共生系统中苯胺降解和脱氮的内在影响，具有不同光周期的 3 组 （0L：12D;6L：6D;12L：0D） 的结果表明，3种体系的苯胺降解率均超过99 %，Z2-6L：6D的总脱氮率比Z1-0L：12D高约36 %，最终Z1-0L：12D被NH4+-N同化和硝化抑制，而Z3-12L：0D被缺氧反硝化。微藻生物量的消失伴随着 Z1 中多糖的急剧减少，较长的光照抑制了细胞外聚合物物质的分泌，尽管与 Z2 相比，尽管双重光照，Z3 的生物量产量略高。此外，高通量测序分析表明，Z2 中的微生物群落结构比 Z3 更丰富，甚至更均匀，Z2 中的 TM7a 、 norank_f__norank_o__Saccharimonadales 、 Ellin6067 和 Scenedesmus 大量增殖，对功能属的光抑制得到有效缓解。