The primary challenge of applying partial denitrification/anammox (PD/A) to municipal wastewater treatment lied in the enrichment of functional bacteria with a considerable autotrophic nitrogen removal performance. The results showed influent NO3−-N: NH4+-N, reaction time and temperature would influence anammox nitrogen removal contribution. 15N isotopic tracing technology further revealed the average anammox contribution rate was up to 94.8 %. Extending reaction time was an effective measure to improve simultaneously PD and anammox activity. Microbial community indicated partial denitrifying bacteria (Bacillus) and anammox bacteria (Candidatus Brocadia) were enriched with abundance of 27.27 % and 7.09 % at NO3−-N: NH4+-N of 1:1. The correlation analysis showed that NO3−-N: NH4+-N ratio played the positive role for Bacillus enrichment, and low temperature was favorable to the enrichment of Thauera and Candidatus Jettenia. Overall, this study demonstrated the reasonable operational strategy would strengthen anammox contribution and facilitate enrichment of functional bacteria.

中文翻译：

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在部分反硝化/厌氧氨氧化系统中实现有效的厌氧氨氧化贡献和功能菌的富集：性能、微生物进化和相关性分析


将部分反硝化/厌氧氨氧化 （PD/A） 应用于城市污水处理的主要挑战在于富含具有相当自养氮去除性能的功能性细菌。结果表明，进水 NO3−-N：NH4+-N、反应时间和反应温度会影响厌氧氨氧化脱氮的贡献。15N 同位素示踪技术进一步显示，平均厌氧氨氧化贡献率高达 94.8%。延长反应时间是同时提高 PD 和厌氧氨氧化活性的有效措施。微生物群落表明部分反化细菌 （Bacillus） 和厌氧氨氧化菌 （Candidatus Brocadia） 在 NO3−-N： NH4+-N 为 1：1 时富集量分别为 27.27 % 和 7.09 %。相关性分析表明，NO3−-N：NH4+-N 比值对芽孢杆菌的富集起积极作用，低温有利于 Thauera 和 Candidatus Jettenia 的富集。总体而言，本研究证明合理的操作策略将加强厌氧氨氧化的作用并促进功能菌的富集。