The accumulation of the emerging pollutant salicylic acid (SA) in the environment has gained much attention. In this study, overexpression of the non-ribosomal peptide synthase (NRPS) gene, PtNRPS1 in the marine diatom Phaeodactylum tricornutum (PtNRPS1-OE) increased resistance to SA pollutants. It was assumed that the enhanced tolerance was due to the high binding affinity between recombinant PtNRPS1 (rNRPS1) and SA pollutants. Moreover, tandem mass spectrometry analysis determined the amino acids that participated in the covalently binding of SA. The removal efficiency of SA pollutants by PtNRPS1-OE cells was found to be markedly elevated. The mechanism underlying the removal of SA and 5-substituted SA (5-sSA) was proposed, following the co-localization analysis of rNRPS1 and SA. The purpose of this study was not about using PtNRPS1 as an enzyme to catalyze the synthesis of metabolite. Rather, it explored the possibility of using PtNRPS1 to remove pollutants, which further improves practical feasibility of microalgae-mediated bioremediation.

中文翻译：

---

PtNRPS1 的过表达增强了硅藻介导的水杨酸盐污染生物修复


新出现的污染物水杨酸 （SA） 在环境中的积累引起了广泛关注。在这项研究中，非核糖体肽合酶 （NRPS） 基因 PtNRPS1 在海洋硅藻 Phaeodactylum tricornutum （PtNRPS1-OE） 中的过表达增加了对 SA 污染物的抵抗力。据推测，增强的耐受性是由于重组 PtNRPS1 （rNRPS1） 和 SA 污染物之间的高结合亲和力。此外，串联质谱分析确定了参与 SA 共价结合的氨基酸。发现 PtNRPS1-OE 细胞对 SA 污染物的去除效率显着升高。在 rNRPS1 和 SA 的共定位分析之后，提出了去除 SA 和 5 取代的 SA （5-sSA） 的潜在机制。本研究的目的不是使用 PtNRPS1 作为催化代谢物合成的酶。相反，它探索了使用 PtNRPS1 去除污染物的可能性，这进一步提高了微藻介导的生物修复的实际可行性。