Food wastedigestatewas fed into a sequencing batch reactor (SBR) forHaloferax mediterranei(HM) to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). This SBR was operated uninterruptedly for 450 days to test its stability, during which the cycle time and volume exchange ratiowere varied to understand their impacts on the PHBV fermentation performance under ranged organic loading rates (OLR). Results showed that 1) PHBV productivity was proportional to OLR of food wastedigestate; 2) substrate and product inhibitions were two limiting factors constraining substrate utilization and PHBV yields; 3) PHBV titer was dependent on the hydraulic retention time of the SBR while a volume exchange ratio lower than 0.5 is unfavorable due to the product inhibitor accumulation. This study for the first time demonstrated that the long-term stability of food waste-fed PHBV production byHMand revealed that inhibition effects could be barriers in SBR limiting the full-scale application of the technology.

中文翻译：

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循环时间和体积交换比对在序批式反应器中培养 450 天的 Haloferax mediterranei 从餐厨垃圾沼渣中生产聚（3-羟基丁酸酯-co-3-羟基戊酸酯）的长期影响


将餐厨垃圾消化液送入地中海卤藻 （HM） 的序批式反应器 （SBR） 中，生产聚（3-羟基丁酸酯-co-3-羟基戊酸酯） （PHBV）。该 SBR 不间断运行 450 天以测试其稳定性，在此期间，周期时间和体积交换比发生变化，以了解它们在范围内有机负荷率 （OLR） 下对 PHBV 发酵性能的影响。结果表明：1） PHBV 生产力与食物垃圾消化物的 OLR 成正比;2） 底物和产物抑制是限制底物利用和 PHBV 产量的两个限制因素;3） PHBV 滴度取决于 SBR 的水力保留时间，而低于 0.5 的体积交换比由于产物抑制剂积累而不利。这项研究首次证明了HMand 生产食物垃圾喂养 PHBV 的长期稳定性，并揭示了抑制作用可能是 SBR 的障碍，限制了该技术的全面应用。