Biochar supplementation could facilitate microbial colonization and increase biogas and methane yield in anaerobic digestion (AD). This study investigated the impact of corn stover biochar (CSB) augmentation on the continuous pilot scale AD of rice straw. The CSB supplementation exhibited the daily average specific biogas and methane yield of 368.6 L/kg volatile solids (VS) and 230 L/kg VS, which were 35% and 37% higher than the control. Principle component analysis indicated that the VS reduction positively correlated with the daily average specific biogas and methane yield. Metagenomic analysis revealed that the CSB supplementation facilitated microbial colonization with the enrichment of unclassified genera from families Planococcaceae, Clostridiaceae, and Ruminococcaceae, and genus Clostridium and methanogenic archaea (Methanosarcina and Methanobacterium). The co-occurrence network revealed a notable shift in microbial interactions following the supplementation of CSB as an additive in AD. These results offer insights for enhancing the performance of the AD using CSB.

中文翻译：

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研究玉米秸秆生物炭补充剂对连续中试规模厌氧消化的作用：性能和微生物群落动力学


生物炭补充剂可以促进微生物定植，并增加厌氧消化 （AD） 中的沼气和甲烷产量。本研究调查了玉米秸秆生物炭 （CSB） 添加对稻草连续中试规模 AD 的影响。CSB 添加的日平均比沼气和甲烷产量分别为 368.6 L/kg 挥发性固体 （VS） 和 230 L/kg VS，分别比对照高 35% 和 37%。主成分分析表明，VS 减少与日平均比沼气和甲烷产量呈正相关。宏基因组分析显示，CSB 补充剂通过富集来自浮球菌科、梭状芽胞杆菌科和瘤胃球菌科以及梭菌属和产甲烷古细菌（甲烷菌属和甲烷杆菌属）的未分类属来促进微生物定植。共生网络揭示了在 AD 中补充 CSB 作为添加剂后微生物相互作用的显着变化。这些结果为使用 CSB 提高 AD 的性能提供了见解。