In this study, we successfully generated the mutant strain Bacillus tropicus AT31-1 from AT31 through atmospheric room-temperature plasma mutagenesis. This mutant strain AT31-1 demonstrated an impressive 48.6 % removal efficiency in 400 mg/L lead medium. Comparative genomic analysis showed that the mutant strain AT31-1 had three mutation sites, which affect the efflux RND transporter permease subunit, the response regulator transcription factor, and a gene with unknown function. The transcriptional analysis showed a notable upregulation in the expression of 283 genes in AT31-1 as lead concentrations increased from 0 to 200 mg/L and then to 400 mg/L, which include zinc-transporting ATPase, ferrous iron transport protein B, NADH dehydrogenase, and others. The Gene ontology function of the peptide metabolic process, along with the KEGG pathway of carbon metabolism were identified as closely linked to the extreme lead tolerance of AT31-1. This study presents novel insights into the lead tolerance mechanisms of bacteria.

中文翻译：

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普洱根际土壤高耐铅突变体热带芽孢杆菌 AT31-1 及其修复机制


在这项研究中，我们通过常压室温血浆诱变成功地从 AT31 中产生了突变菌株热带芽孢杆菌 AT31-1。该突变菌株 AT31-1 在 400 mg/L 铅培养基中表现出令人印象深刻的 48.6% 去除效率。比较基因组分析显示，突变菌株 AT31-1 具有 3 个突变位点，影响外排 RND 转运蛋白通透酶亚基、反应调节因子转录因子和一个功能未知的基因。转录分析显示，当铅浓度从 0 增加到 200 mg/L，然后增加到 400 mg/L 时，AT31-1 中 283 个基因的表达显着上调，其中包括锌转运 ATP 酶、亚铁转运蛋白 B、NADH 脱氢酶等。肽代谢过程的基因本体功能以及碳代谢的 KEGG 通路被确定与 AT31-1 的极铅耐受性密切相关。本研究为细菌的铅耐受性机制提供了新的见解。