Recombinant DNA technology has profoundly advanced virtually every aspect of biological and medical sciences, from basic research to biotechnology. Here, I discuss conceptual connections linking fundamental discoveries that were enabled by the technology, advances in the understanding of gene regulation in both prokaryotes and eukaryotes, and the recent FDA-approved CRISPR-based gene therapy for sickle cell anemia and β-thalassemia based on transcriptional derepression.

中文翻译：

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从细菌操纵子到基因治疗：《细胞》杂志 50 周年


重组 DNA 技术几乎深刻地推动了生物和医学科学的各个方面，从基础研究到生物技术。在这里，我讨论了将该技术实现的基础发现、对原核生物和真核生物基因调控的理解进展以及最近 FDA 批准的基于 CRISPR 的基因疗法联系起来的基于转录抑制的镰状细胞贫血和β地中海贫血。