Human milk oligosaccharides are of high interest as active ingredients in infant formulas and dietary food supplements. Full characterization of members of this compound class is challenging due to the intrinsic complexity of byproducts during synthesis by fermentation. Moreover, when method validation is targeted for a regulated environment, a robust chromatographic separation of the highly polar oligosaccharides needs to be addressed, including isomers and compounds relevant for potential product adulteration. We present a combined approach of validated chromatography and NMR spectroscopy, which allows for full mass balancing of industrially produced 2′-fucosyl-d-lactose. A combination of NMR spectroscopy, mass spectrometry, and action IR spectroscopy tackles structural elucidation of monoacetylated species as a new class of byproducts.

中文翻译：

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用于全面表征 2′-岩藻糖基-d-乳糖作为工业生产母乳低聚糖的经过验证的多方法方法


母乳低聚糖作为婴儿配方奶粉和膳食食品补充剂中的活性成分备受关注。由于发酵合成过程中副产物的内在复杂性，因此对这类化合物的成员进行全面表征具有挑战性。此外，当方法验证针对受监管环境时，需要解决高极性低聚糖的稳定色谱分离问题，包括与潜在产品掺假相关的异构体和化合物。我们提出了一种经过验证的色谱和 NMR 波谱的组合方法，该方法允许工业生产的 2′-岩藻糖基-d-乳糖的完全质量平衡。NMR 波谱、质谱和作用红外光谱相结合，解决了单乙酰化物质作为一类新型副产物的结构解析问题。