Sclerotium rolfsii is an extremely destructive phytopathogenic fungus that causes significant economic losses. Biocontrol strategies utilizing antagonistic microorganisms present a promising alternative for controlling plant pathogens. Bacillus megaterium L2 has been identified as a potential microbial biocontrol agent in our previous study; however, its efficacy in controlling pathogens has yet to meet current demands. This study aims to enhance the antifungal activity of strain L2 against S. rolfsii R-67 through a two-round mutagenesis strategy and to preliminarily investigate the mutagenesis mechanism of the high antifungal activity mutant.

中文翻译：

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常温和室温等离子体与核糖体工程技术相结合，以增强巨型芽孢杆菌 L2 对 Sclerotium rolfsii 的抗真菌活性


Sclerotium rolfsii 是一种极具破坏性的植物病原真菌，造成重大经济损失。利用拮抗微生物的生物防治策略为控制植物病原体提供了一种很有前途的替代方案。巨型芽孢杆菌在我们之前的研究中，L2 已被确定为潜在的微生物生物防治剂;然而，它在控制病原体方面的功效尚未满足当前的需求。本研究旨在通过两轮诱变策略增强菌株 L2 对 S. rolfsii R-67 的抗真菌活性，并初步研究高抗真菌活性突变体的诱变机制。