Reactivity-based screening (RBS) was used to screen fungal crude extracts for the presence of new azaphilic natural products. A probe composed of a nucleophilic primary amine and an isotopic mass tag was designed for its reactivity towards azaphilic compounds. Addition of the probe to crude extracts of fungal complexes, together with analytical tools for dereplication such as haloseeker and molecular networking, allowed easy detection of azaphilic compounds. The exploitation of this RBS protocol led to the isolation and structural characterization of new azaphilones.

中文翻译：

---

用于快速发现真菌粗提取物中嗜氮天然产物的质谱标记胺化探针


基于反应性的筛选 （RBS） 用于筛选真菌粗提取物中是否存在新的嗜氮天然产物。由亲核伯胺和同位素质量标签组成的探针被设计用于其对亲氮化合物的反应性。将探针添加到真菌复合物的粗提取物中，以及用于去重的分析工具，例如 haloseeker 和分子网络，可以轻松检测嗜氮化合物。该 RBS 方案的开发导致了新 azaphilones 的分离和结构表征。