Developing instant detection systems with disease diagnostic capabilities holds immense importance for remote or resource-limited areas. However, the task of creating these systems—which are simultaneously easy to operate, rapid in detection, and cost-effective—remains a challenge. In this study, we present a compact highly sensitive photothermal reverse transcriptase–loop-mediated isothermal amplification (RT-LAMP) chip (SPRC) designed for the detection of multiple diseases. The nucleic acid (NA) amplification on the chip is achieved through LAMP driven by either LED illumination or simple sunlight focusing. SPRC performs sample addition and amplification within a limited volume and autonomous enrichment of NA during the sample addition process, achieving a limit of detection (LOD) as low as 0.2 copies per microliter. Through 120 clinical samples, we achieved an accuracy of 95%, with a specificity exceeding 97.5%. Overall, SPRC has achieved promising progress in the application of point-of-care testing (POCT) by using light energy to simultaneously detect multiple diseases.

中文翻译：

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用于同时检测多种疾病的紧凑型高灵敏度光热 RT-LAMP 芯片


开发具有疾病诊断能力的即时检测系统对于偏远或资源有限的地区具有极其重要的意义。然而，创建这些系统的任务仍然是一项挑战，这些系统同时易于操作、检测快速且具有成本效益。在这项研究中，我们提出了一种紧凑的高灵敏度光热逆转录酶-环介导的等温扩增 （RT-LAMP） 芯片 （SPRC），旨在检测多种疾病。芯片上的核酸 （NA） 扩增是通过 LED 照明或简单的阳光聚焦驱动的 LAMP 实现的。SPRC 在有限体积内进行样品添加和扩增，并在样品添加过程中自主富集 NA，实现低至 0.2 拷贝/μL 的检测限 （LOD）。通过 120 个临床样本，我们实现了 95% 的准确率，特异性超过 97.5%。总体而言，SPRC 通过使用光能同时检测多种疾病，在即时检测 （POCT） 的应用方面取得了可喜的进展。