The most common methods for multiplexed immunohistochemistry rely on cyclic procedures, whereby cells or tissues are repeatedly stained, imaged, and regenerated. Here, we present a simple and inexpensive approach for amine-targeted labeling of antibodies using a linker that can be easily cleaved by a mild reducing agent. This method requires only inexpensive and readily-available reagents, and can be carried out without synthetic experience in a simple one-pot reaction. We demonstrate the applicability of this approach by performing repeated staining-imaging-removal cycles on isolated cells and tissue sections, finding that over 94% of the labels can be removed within 30 minutes using only the gentle application of reducing agent, increasing up to 99% by extending the incubation duration to 1 hour. By providing a convenient way to introduce cleavable linkers, our method simplifies methodologies such as high-content imaging or multiplexed immunohistochemistry.

中文翻译：

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轻松生成用于多重成像的可切割标签


多重免疫组织化学的最常见方法依赖于循环程序，其中细胞或组织被反复染色、成像和再生。在这里，我们提出了一种简单且廉价的抗体胺靶向标记方法，使用可以轻松被温和还原剂裂解的接头。该方法只需要廉价且容易获得的试剂，并且无需合成经验即可在简单的一锅反应中进行。我们通过在分离的细胞和组织切片上进行重复的染色-成像-去除循环来证明这种方法的适用性，发现仅使用温和的还原剂即可在 30 分钟内去除超过 94% 的标记，通过将孵育时间延长至 99 小时，最多可增加 1%。通过提供一种方便的方式来引入可切割接头，我们的方法简化了高内涵成像或多重免疫组织化学等方法。