Accurately monitoring the freshness of high-protein foods has significant implications for both food safety and public welfare. Since a large amount of hydrogen sulfide (H2S) is produced during spoilage-related processes, abnormal H2S levels are often considered an important indicator of food spoilage. Therefore, we synthesized novel nanoparticles (NPs) containing Silicon (Si) dots and CdTe quantum dots to accurately assess the amount of sulfide ions (S2−) and thus the quality of high-protein foods in the early stage of storage. As the concentration of S2− increased, the fluorescence intensity of Si/CdTe NPs at λem = 488 nm increased, while the fluorescence intensity at λem = 620 nm was quenched. The fluorescence intensity ratio (F620/F488) was negatively linearly correlated to S2− concentrations in the range of 1–20 μM, with a detection limit of 0.3 μM. Furthermore, to achieve portable detection, we mixed Si/CdTe NPs with sodium carboxymethyl cellulose to prepare effective visual fluorescent sensing paper chips, which exhibited ideal porous structure, good particle dispersion, and excellent fluorescence properties. Incubating the paper chips with high-protein foods allowed for accurate monitoring of food freshness during storage. Therefore, this approach provided a reliable and portable method to determine H2S concentration using a novel concept to ensure the freshness and safety of high-protein foods.

中文翻译：

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用于监测高蛋白食品新鲜度的比率荧光纸芯片


准确监测高蛋白食品的新鲜度对食品安全和公共福利都有重大影响。由于在与腐败相关的过程中会产生大量的硫化氢 （H2S），因此异常的 H2S 水平通常被认为是食品腐败的重要指标。因此，我们合成了含有硅 （Si） 点和 CdTe 量子点的新型纳米颗粒 （NPs），以准确评估硫化物离子 （S2−） 的量，从而评估高蛋白食品在储存早期的质量。随着 S2− 浓度的增加，Si/CdTe NPs 在 λem = 488 nm 处的荧光强度增加，而 λem = 620 nm 处的荧光强度被淬灭。荧光强度比 （F620/F488） 与 S2− 浓度在 1–20 μM 范围内呈负线性相关，检测限为 0.3 μM。此外，为了实现便携式检测，我们将 Si/CdTe NPs 与羧甲基纤维素钠混合制备了有效的视觉荧光传感纸芯片，该芯片表现出理想的多孔结构、良好的颗粒分散性和优异的荧光性能。将纸片与高蛋白食品一起孵育，可以准确监测储存过程中的食物新鲜度。因此，这种方法提供了一种可靠且便携的方法，使用新颖的概念来确定 H2S 浓度，以确保高蛋白食品的新鲜度和安全性。