Fluctuation-based super-resolution microscopy enhances image resolution using signal fluctuations, yet the inherent fluctuations of fluorophores limit its spatiotemporal resolution. In this work, we reveal a far-field enhancement (FFE) effect via a nanosilver film that significantly boosts fluorescence fluctuations of fluorophores positioned up to 10 μm away. The FFE effect arises from the interference of scattered light from the nanosilver film and photothermal-induced refractive index changes in the imaging medium, which create periodic auxiliary illumination on the sample. This phenomenon enabled the development of far-field enhanced super-resolution microscopy (FFE-SRM), a technique compatible with commonly used fluorophores. FFE-SRM improves temporal resolution up to 10-fold and enhances spatial resolution by about 2-fold over various SRM methods, including stochastic optical fluctuation imaging, super-resolution radial fluctuation, mean-shift super-resolution, and direct stochastic optical reconstruction microscopy. We demonstrated the potential of FFE-SRM by revealing mitochondrial dynamics in live-cell imaging, advancing super-resolution imaging, and cellular process exploration.

中文翻译：

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用于超分辨率显微镜的纳米银增强远场荧光波动


基于波动的超分辨率显微镜利用信号波动提高图像分辨率，但荧光团的固有波动限制了其时空分辨率。在这项工作中，我们揭示了通过纳米银膜的远场增强 （FFE） 效应，该效应显着增强了位于 10 μm 外的荧光团的荧光波动。FFE 效应是由纳米银膜的散射光和成像介质中光热诱导的折射率变化的干扰引起的，这些变化会在样品上产生周期性的辅助照明。这种现象促成了远场增强超分辨率显微镜 （FFE-SRM） 的发展，这是一种与常用荧光团兼容的技术。与各种 SRM 方法相比，FFE-SRM 将时间分辨率提高了 10 倍，空间分辨率提高了约 2 倍，包括随机光学波动成像、超分辨率径向波动、均值偏移超分辨率和直接随机光学重建显微镜。我们通过揭示活细胞成像中的线粒体动力学、推进超分辨率成像和细胞过程探索来证明 FFE-SRM 的潜力。