Aminoglycoside antibiotics (AGs) are commonly utilized in both human and veterinary medicine to treat and manage a range of infections. These antibiotics are recognized for their narrow therapeutic window, with an overdose potentially resulting in severe side effects like kidney and ear damage. Hence, the implementation of a quick, precise, and on-the-spot testing method is crucial in clinical settings. In the present investigation, we designed an innovative indirect lateral flow assay (LFA) utilizing aptamers to detect kanamycin, a type of aminoglycoside antibiotics. We prepared mesoporous silica nanoparticles (MSNs) functionalized with amino groups, loaded them with morphine (MOP), and then sealed them with aminoglycoside aptamers (Apt). The complex of gated-MSNs@Apt was tested using the MOP LFA in the presence or absence of kanamycin antibiotics.

中文翻译：

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一种创新的生物传感器，利用适配体门控介孔二氧化硅纳米颗粒通过间接侧向流动测定氨基糖苷类抗生素


氨基糖苷类抗生素 （AGs） 通常用于人类和兽医学，以治疗和管理一系列感染。这些抗生素因其治疗窗口狭窄而著称，过量服用可能会导致严重的副作用，如肾脏和耳朵损伤。因此，在临床环境中实施快速、精确和现场的检测方法至关重要。在本研究中，我们设计了一种创新的间接侧向层析测定 （LFA），利用适配体检测卡那霉素，一种氨基糖苷类抗生素。我们制备了用氨基官能化的介孔二氧化硅纳米颗粒 （MSN），用吗啡 （MOP） 加载它们，然后用氨基糖苷适配体 （Apt） 密封它们。在存在或不存在卡那霉素抗生素的情况下使用 MOP LFA 测试门控MSNs@Apt复合物。