P-bodies contain a variety of RNA and RNA-binding proteins (RBPs) and modulate their RNA content in response to stress. However, changes in P-body composition in unstressed conditions is less well studied.

In recent work, using RNA sequencing and single-molecule fluorescence in situ hybridization, Safieddine et al. show that mRNA localization in P-bodies is highly dynamic during the cell cycle. P-bodies enlarge during cell cycle progression, but their proteome remains mostly unchanged. Further analysis showed that RNA accumulation in P-bodies is partly uncoupled from their cytoplasmic abundance. Cyclic mRNA localization to P-bodies does not result from fluctuating amounts of non-polysomal mRNAs. The authors identified human antigen R (HuR) as an RBP that contributes to cyclic mRNA localization by preventing their recruitment in P-bodies during G2. Searching for mRNA features that participate in P-body localization, the researchers found that the mRNA nucleotide composition (GC-rich) and the mRNA length modulate cell cycle-dependent localization in P-bodies.

P 体包含多种 RNA 和 RNA 结合蛋白 （RBP），并在应激下调节其 RNA 含量。然而，在无应力条件下 P 体组成的变化研究较少。

在最近的工作中，使用 RNA 测序和单分子荧光原位杂交，Safieddine 等人表明 M RNA 在 P 体中的定位在细胞周期中是高度动态的。P 体在细胞周期进程中增大，但其蛋白质组基本保持不变。进一步分析表明，P 体中的 RNA 积累与其细胞质丰度部分脱钩。环状 mRNA 定位到 P 体不是由非多核糖体 mRNA 量波动引起的。作者将人抗原 R （HuR） 确定为一种 RBP，通过在 G2 期间阻止它们在 P 体中募集来促进环状 mRNA 定位。通过寻找参与 P 体定位的 mRNA 特征，研究人员发现 mRNA 核苷酸组成（富含 GC）和 mRNA 长度调节 P 体中的细胞周期依赖性定位。