Blackcurrant (Ribes nigrum L., family Grossulariaceae) is a perennial shrub that is widely cultivated for its edible berries. These are rich in antioxidants, vitamin C and anthocyanins, making them a valuable ingredient in the food and beverage industry. However, prolonged periods of drought during the fruiting season lead to drought stress, which has serious ecological and agricultural implications, inhibiting blackcurrant growth and reducing yields. To facilitate the analysis of underlying molecular processes, we present the first high-quality chromosome-scale and partially haplotype-resolved assembly of the blackcurrant genome (cv. Rosenthals Langtraubige), also the first in the family Grossulariaceae. We used this genomic reference to analyze the transcriptomic response of blackcurrant leaves and roots to drought stress, revealing differentially expressed genes with diverse functions, including those encoding the transcription factors bZIP, bHLH, MYB and WRKY, and tyrosine kinase-like kinases such as PERK and DUF26. Gene expression was correlated with the abundance of primary metabolites, revealing 14 with significant differences between stressed leaves and controls indicating a metabolic response to drought stress. Amino acids such as proline were more abundant under stress conditions, whereas organic acids were depleted. The genomic and transcriptomic data from this study can be used to develop more robust blackcurrant cultivars that thrive under drought stress conditions.

中文翻译：

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全基因组组装揭示了干旱胁迫对黑醋栗 （Ribes nigrum L.） 基因表达和代谢物谱的影响。


黑醋栗 （Ribes nigrum L.，Grossulariaceae 科） 是一种多年生灌木，因其可食用浆果而被广泛种植。它们富含抗氧化剂、维生素 C 和花青素，使其成为食品和饮料行业的宝贵成分。然而，果季的长期干旱会导致干旱胁迫，这对生态和农业有严重的影响，抑制黑醋栗的生长并降低产量。为了便于分析潜在的分子过程，我们提出了黑醋栗基因组 （cv. Rosenthals Langtraubige） 的第一个高质量染色体规模和部分单倍型分辨的组装，也是 Grossulariaceae 家族的第一个。我们使用此基因组参考来分析黑醋栗叶片和根对干旱胁迫的转录组响应，揭示了具有不同功能的差异表达基因，包括编码转录因子 bZIP 、 bHLH 、 MYB 和 WRKY 的基因，以及酪氨酸激酶样激酶 如 PERK 和 DUF26。基因表达与初级代谢物的丰度相关，显示 14 个在胁迫叶片和对照之间具有显着差异，表明对干旱胁迫的代谢反应。脯氨酸等氨基酸在胁迫条件下更丰富，而有机酸则耗尽。这项研究的基因组和转录组数据可用于开发在干旱胁迫条件下茁壮成长的更健壮的黑醋栗栽培品种。