ScopeMetabolic dysfunction‐associated steatotic liver disease (MASLD) is a global health issue with increasing prevalence. Polyphenols, such as grape seed proanthocyanidin extract (GSPE), are bioactive compounds present in plants and represent an interesting therapeutical approach for MASLD.Methods and resultsThis study questioned whether the timing of GSPE administration impacts liver diurnal metabolism and steatosis in a rat obesity model. Results from hepatic lipid profiling and diurnal metabolic gene expression and metabolomics reveal that rats fed with a cafeteria (CAF) diet show impaired glucose homeostasis and enhanced lipogenesis in the liver, contributing to liver steatosis. Chronic consumption of GSPE in the inactive or active phase is associated with beneficial effects as the restoration of rhythms of transcripts and metabolites is observed. However, only when given in the active phase, GSPE treatment decreases hepatic triglyceride levels. Using an in vitro hepatocyte model, the study identifies that catechin, one of the main phenolic compounds found in the GSPE extract, is a potential mediator in ameliorating the effects of CAF‐induced liver steatosis.ConclusionTaken altogether, the findings show that the beneficial effects of GSPE on MASLD development depend on the treatment time.

中文翻译：

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葡萄籽原花青素提取物 （GSPE） 调节肝脏代谢基因和代谢物的昼夜节律，并以一天中的时间依赖性方式减少食堂喂养大鼠体内的脂质沉积


范围代谢功能障碍相关脂肪性肝病 （MASLD） 是一个全球性的健康问题，其患病率不断上升。多酚，如葡萄籽原花青素提取物 （GSPE），是存在于植物中的生物活性化合物，代表了 MALD 的一种有趣的治疗方法。方法和结果本研究质疑 GSPE 给药的时间是否会影响大鼠肥胖模型中的肝脏昼夜代谢和脂肪变性。肝脂质分析和昼夜代谢基因表达和代谢组学的结果表明，用自助餐厅 （CAF） 饮食喂养的大鼠表现出葡萄糖稳态受损和肝脏脂肪生成增强，导致肝脏脂肪变性。在非活性或活性期长期食用 GSPE 与有益效果相关，因为观察到转录物和代谢物节律的恢复。然而，只有在活性期给药时，GSPE 治疗才能降低肝脏甘油三酯水平。使用体外肝细胞模型，该研究确定儿茶素是 GSPE 提取物中发现的主要酚类化合物之一，是改善 CAF 诱导的肝脂肪变性影响的潜在介质。结论总而言之，研究结果表明 GSPE 对 MASLD 发展的有益影响取决于治疗时间。