Herbicide-metolachlor (MA) residue seriously pollutes the environment and endangers human health. A novel electrochemical immunosensor featuring the surface-adhered nanofiber-particle films has been developed for the specific detection of metolachlor. The three-dimensional nanofiber-particle films, fabricated through the combination of nanomaterial modification techniques, including alkali treatment of polyethylene terephthalate (PET) and carboxylation of graphene oxide (GO), along with electrospinning technology, served as the platform for the immobilization of the catalyst p-Phenylenediamine (pPd) and the reactant metolachlor antibody (MAb). The sensor exhibited a satisfactory linear detection range (2.00 to 30.00 ng mL−1), a low limit of detection (LOD, 0.17 ng mL−1), and good repeatability and stability (detection activity ≥ 90.00 % after 10.00 uses/storage for 10.00 days, remaining at 80.00 % after 30 uses/storage). Furthermore, it demonstrated acceptable recovery rates (89.73 % to 92.78 %) in real sample detection. In the future, our sensor is anticipated to play a crucial role in detecting MA residues in crops.

中文翻译：

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一种基于 PET/GO/pPd/MAb 纳米纤维颗粒的高灵敏度电化学免疫传感器，用于异丙甲草胺检测


除草剂异丙甲草胺 （MA） 残留严重污染环境，危害人类健康。已经开发了一种具有表面粘附纳米纤维颗粒膜的新型电化学免疫传感器，用于异丙甲草胺的特异性检测。通过结合纳米材料改性技术制造的三维纳米纤维颗粒薄膜，包括聚对苯二甲酸乙二醇酯 （PET） 的碱处理和氧化石墨烯 （GO） 的羧化，以及静电纺丝技术，作为催化剂对苯二胺 （pPd） 和反应物异丙甲草胺抗体 （MAb） 固定化的平台。该传感器表现出令人满意的线性检测范围（2.00 至 30.00 ng mL-1）、低检测限（LOD，0.17 ng mL-1）以及良好的可重复性和稳定性（检测活性≥ 90.00 %（10.00 次使用/储存 10.00 天后检测活性为 90.00 %，每次储存 30 次后仍为 80.00 %））。此外，在实际样品检测中，它显示出可接受的回收率 （89.73% 至 92.78 %）。未来，我们的传感器有望在检测农作物中的 MA 残留物方面发挥关键作用。