The presence of 2-methylisoborneol (2-MIB) is acknowledged as a prevalent source of odor-related challenges in drinking water reservoirs. Among the three in situ experiments conducted in drinking water reservoir, the water-lifting aerator with bio-filling system exhibited the most pronounced overall effects. It achieved a remarkable 98.70 % removal of 2-MIB and a 99.30 % reduction in the abundance of the mic gene. Metagenomic sequencing identified key genes including methyl transferase gene (mtf), 2-MIB cyclase gene (mic), cyclic nucleotide-binding protein gene (cnb), underscoring the potential role of Actinobacteria, Cyanobacteria, and Proteobacteria communities in contributing to odor occurrences in the reservoir. Furthermore, the abundance of odorous precursor enzymes in the MVA pathway and MEP/DOXP pathway were inhibited in the systems with bio-filling. Total nitrogen (TN) and nitrate (NO₃⁻-N) were identified as pivotal factors influencing the presence of 2-MIB odor-producing microorganisms. Effective management of odor-producing species in reservoir water was closely related to the efficient removal of pollutants. These findings will provide valuable insights for the development of odor removal techniques in reservoirs and offer researchers deeper understanding into the mechanisms underlying odor processes.

中文翻译：

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饮用水储层中气味的显著下降：气味群落的变化、前体酶的丰度、分布和环境主导因素


2-甲基异冰片 （2-MIB） 的存在被认为是饮用水水库中与气味相关的挑战的普遍来源。在饮用水水库进行的 3 次原位实验中，采用生物充注系统的提水增氧机整体效果最为显著。它实现了 98.70% 的 2-MIB 去除和 99.30% 的 mic 基因丰度降低。宏基因组测序确定了关键基因，包括甲基转移酶基因 （mtf）、2-MIB 环化酶基因 （mic）、环核苷酸结合蛋白基因 （cnb），强调了放线菌门、蓝细菌门和变形菌门群落在导致储层气味发生方面的潜在作用。此外，在生物填充系统中，MVA 通路和 MEP/DOXP 通路中气味前体酶的丰度受到抑制。总氮 （TN） 和硝酸盐 （NO₃⁻-N） 被确定为影响 2-MIB 产生气味的微生物存在的关键因素。有效管理水库水中产生气味的物种与有效去除污染物密切相关。这些发现将为储层除臭技术的发展提供有价值的见解，并为研究人员更深入地了解气味过程的潜在机制。