BACKGROUNDCucumber Fusarium wilt (CFW), triggered by Fusarium oxysporum f. sp. cucumerinum, leads to substantial yield reductions in global cucumber (Cucumis sativus L.) production. Common management strategies for CFW include soil fumigation, grafting, and crop rotation. However, these methods have limitations regarding safety and efficacy stability, necessitating the development of new, cost‐effective, and eco‐friendly control strategies. Our prior research demonstrated that L‐arabinose, an inexpensive and safe sugar commonly used in food and beverages, effectively suppressed bacterial wilt in tomatoes. This study explores the potential of L‐arabinose in managing CFW and investigates its mechanism of action.RESULTSSoil applications of L‐arabinose, ranging from 0.00001 to 0.01%, effectively suppressed CFW. The most significant suppressive effect was observed at 0.01%, reducing the disease severity index by 67.5% compared to the control treatment. Microscopic examination of transverse root sections showed that pathogen hyphae colonized the epidermis but seldom penetrated the cortical layer of roots in L‐arabinose‐treated seedlings. In contrast, the entire root tissue of control seedlings was colonized by the pathogen. Quantitative real‐time PCR revealed a significant increase in the expression of defense‐related genes dependent on salicylic acid, jasmonic acid, and ethylene in L‐arabinose‐treated plants compared to control plants, 6 and 10 days post pathogen inoculation.CONCLUSIONThis study demonstrated that soil application of L‐arabinose can effectively suppress CFW by priming root tissues for multiple defense signaling pathways. Therefore, L‐arabinose holds potential as a new fungicide for managing CFW. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

中文翻译：

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L-阿拉伯糖控制黄瓜镰刀菌枯萎病的疗效及作用机制


背景由尖孢镰刀菌 f. sp. 黄瓜引发的镰刀菌枯萎病 （CFW） 导致全球黄瓜 （Cucumis sativus L.） 产量大幅下降。CFW 的常见管理策略包括土壤熏蒸、嫁接和轮作。然而，这些方法在安全性和有效性稳定性方面存在局限性，因此需要开发新的、具有成本效益的和环保的控制策略。我们先前的研究表明，L-阿拉伯糖是一种廉价且安全的糖，常用于食品和饮料，可有效抑制西红柿中的细菌性枯萎病。本研究探讨了 L-阿拉伯糖在管理 CFW 方面的潜力并研究了其作用机制。结果L-阿拉伯糖的土壤施用范围为 0.00001 至 0.01%，有效抑制了 CFW。观察到最显著的抑制效果为 0.01%，与对照治疗相比，疾病严重程度指数降低了 67.5%。横向根切片的显微镜检查表明，病原体菌丝定植于表皮，但很少穿透 L-阿拉伯糖处理的幼苗的根皮质层。相比之下，对照幼苗的整个根组织都被病原体定植。定量实时 PCR 显示，在病原体接种后 6 天和 10 天，与对照植物相比，L-阿拉伯糖处理的植物中依赖于水杨酸、茉莉酸和乙烯的防御相关基因的表达显着增加。结论本研究表明，土壤施用 L-阿拉伯糖可通过启动根组织进行多种防御信号通路来有效抑制 CFW。因此，L-阿拉伯糖具有作为控制 CFW 的新型杀菌剂的潜力。© 2024 作者。 由John Wiley & Sons Ltd代表化学工业协会出版的《害虫管理科学》。