A novel NIR-II cyanine dye denoted as Sulfo-1100 was designed and synthesized to realize high-fidelity in vivo imaging of orthotopic glioma. The dye in FBS (10%) and PBS showed NIR-I absorptions (866 nm and 776 nm, respectively) and NIR-II fluorescence emissions (1114 nm and 1120 nm). To realize specific imaging for orthotopic glioma in vivo, Sulfo-1100 was assembled with holo-Tf to form hT-Sulfo-1100 nanoparticles (NPs). They showed excellent targeting and through-skull imaging ability in as soon as 10 min. More importantly, hT-Sulfo-1100 NPs and free Sulfo-1100 could be excreted through the renal clearance pathway within 12 h and were retained neither in the liver nor spleen, avoiding a long-term toxicity risk. This novel water-soluble NIR-II fluorescent dye offers a tool for deep-tissue through-skull in situ imaging to detect tumors with high optical resolution and good biocompatibility.

中文翻译：

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一种新型水溶性 NIR-II 花青染料，用于原位神经胶质瘤的快速靶向颅腔荧光成像


设计合成了一种名为 Sulfo-1100 的新型 NIR-II 花青染料，以实现原位胶质瘤的高保真体内成像。FBS （10%） 和 PBS 中的染料显示 NIR-I 吸收（分别为 866 nm 和 776 nm）和 NIR-II 荧光发射（1114 nm 和 1120 nm）。为了实现体内原位神经胶质瘤的特异性成像，将 Sulfo-1100 与 holo-Tf 组装形成 hT-Sulfo-1100 纳米颗粒 （NPs）。他们在短短 10 分钟内就表现出了出色的靶向和颅骨成像能力。更重要的是，hT-Sulfo-1100 NPs 和游离 Sulfo-1100 可在 12 h 内通过肾脏清除途径排泄，并且既不保留在肝脏和脾脏中，避免了长期毒性风险。这种新型水溶性 NIR-II 荧光染料为深层组织颅骨原位成像提供了一种工具，以检测具有高光学分辨率和良好生物相容性的肿瘤。