Chiral detection of tryptophan (Trp) isomers is of vital importance and the introduction of efficient, stable and sensitive enantioselective recognition technology is in high demand in the fields of medicine, food and life science. Herein, a facile and sensitive electrochemical probe for the chiral recognition of Trp enantiomers by using chiral polyaniline (D-PANI) and β-CD-MOF is presented. The structure and electrochemical performance of the designed sensor (GCE/β-CD-MOF/D-PANI) were characterized via FT-IR, SEM, TEM, EIS, CV and DPV methods. Under the optimized conditions, the enantiomer selection coefficient (I_L/I_D) reached 2.26, and the detection limits for L- and D-Trp were calculated to be 6.67 and 16.1 μM, respectively. The higher DPV result for L-Trp was attributed to its higher affinity for the chiral selectors D-PANI and β-CD-MOF than that of D-Trp. Moreover, the proposed chiral sensing platform could be used for analyzing the percentage of D-Trp in enantiomer mixed solutions and human urine samples with satisfactory recoveries.

中文翻译：

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使用手性聚苯胺和 β-CD-MOF 电化学手性识别色氨酸对映异构体


色氨酸 （Trp） 异构体的手性检测至关重要，医学、食品和生命科学领域对引入高效、稳定和灵敏的对映选择性识别技术的需求很高。在此，提出了一种简单而灵敏的电化学探针，用于使用手性聚苯胺 （D-PANI） 和 β-CD-MOF 手性识别 Trp 对映异构体。通过 FT-IR、SEM、TEM、EIS、CV 和 DPV 方法表征了所设计的传感器 （GCE/β-CD-MOF/D-PANI） 的结构和电化学性能。在优化条件下，对映异构体选择系数 （I_L/I_D） 达到 2.26，计算出 L- 和 D-Trp 的检测限分别为 6.67 和 16.1 μM。L-Trp 的 DPV 结果较高，这是由于其对手性选择因子 D-PANI 和 β-CD-MOF 的亲和力高于 D-Trp。此外，所提出的手性传感平台可用于分析对映异构体混合溶液和人尿液样品中 D-Trp 的百分比，并得到满意的回收率。